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TFB1M antibody - N-terminal region

Rabbit Polyclonal Antibody

     
  • IHC - TFB1M antibody - N-terminal region AI10684
    Rabbit Anti-TFB1M Antibody
    Catalog Number: AI10684
    Formalin Fixed Paraffin Embedded Tissue: Human Adult heart Observed Staining: Cytoplasmic
    Primary Antibody
    Concentration: 1:600
    Secondary Antibody: Donkey anti-Rabbit-Cy2/3
    Secondary Antibody
    Concentration: 1:200
    Magnification: 20X
    Exposure Time: 0.5 – 2.0 sec
    Protocol located in Reviews and Data.
  • IHC - TFB1M antibody - N-terminal region AI10684
    Rabbit Anti-TFB1M Antibody
    Catalog Number: AI10684
    Formalin Fixed Paraffin Embedded Tissue: Human Adult liver Observed Staining: Cytoplasmic
    Primary Antibody
    Concentration: 1:100
    Secondary Antibody: Donkey anti-Rabbit-Cy2/3
    Secondary Antibody
    Concentration: 1:200
    Magnification: 20X
    Exposure Time: 0.5 – 2.0 sec
    Protocol located in Reviews and Data.
  • WB - TFB1M antibody - N-terminal region AI10684

    WB Suggested Anti-TFB1M Antibody Titration: 0.2-1 μg/ml
    ELISA Titer: 1:312500
    Positive Control: Human Liver
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB
Primary Accession Q8WVM0
Other Accession NM_016020, NP_057104
Reactivity Human, Rat, Rabbit, Zebrafish, Horse, Bovine, Dog
Predicted Pig, Chicken, Horse, Bovine
Host Rabbit
Clonality Polyclonal
Calculated MW 39kDa
Additional Information
Gene ID 51106
Alias Symbol CGI-75, CGI75, mtTFB, mtTFB1
Other Names Dimethyladenosine transferase 1, mitochondrial, 2.1.1.-, Mitochondrial 12S rRNA dimethylase 1, Mitochondrial transcription factor B1, h-mtTFB, h-mtTFB1, hTFB1M, mtTFB1, S-adenosylmethionine-6-N', N'-adenosyl(rRNA) dimethyltransferase 1, TFB1M
Format Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Reconstitution & Storage Add 50 ul of distilled water. Final anti-TFB1M antibody concentration is 1 mg/ml in PBS buffer with 2% sucrose. For longer periods of storage, store at 20°C. Avoid repeat freeze-thaw cycles.
PrecautionsTFB1M antibody - N-terminal region is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name TFB1M
Function S-adenosyl-L-methionine-dependent methyltransferase which specifically dimethylates mitochondrial 12S rRNA at the conserved stem loop. Also required for basal transcription of mitochondrial DNA, probably via its interaction with POLRMT and TFAM. Stimulates transcription independently of the methyltransferase activity.
Cellular Location Mitochondrion.
Tissue Location Ubiquitously expressed.
Research Areas
Citations (0)

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References

Cotney,J., (2007) Nucleic Acids Res. 35 (12), 4042-4054 Reconstitution and Storage:For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.

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$ 293.00
Cat# AI10684
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