HELB antibody - N-terminal region
Rabbit Polyclonal Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| WB |
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Primary Accession | Q8NG08 |
Other Accession | NM_033647, NP_387467 |
Reactivity | Human, Rat, Pig, Bovine |
Predicted | Human, Pig |
Host | Rabbit |
Clonality | Polyclonal |
Calculated MW | 120kDa |
Gene ID | 92797 |
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Alias Symbol | hDHB |
Other Names | DNA helicase B, hDHB, 3.6.4.12, HELB |
Format | Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose. |
Reconstitution & Storage | Add 100 ul of distilled water. Final anti-HELB antibody concentration is 1 mg/ml in PBS buffer with 2% sucrose. For longer periods of storage, store at 20°C. Avoid repeat freeze-thaw cycles. |
Precautions | HELB antibody - N-terminal region is for research use only and not for use in diagnostic or therapeutic procedures. |
Name | HELB (HGNC:17196) |
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Function | 5'-3' DNA helicase involved in DNA damage response by acting as an inhibitor of DNA end resection (PubMed:25617833, PubMed:26774285). Recruitment to single-stranded DNA (ssDNA) following DNA damage leads to inhibit the nucleases catalyzing resection, such as EXO1, BLM and DNA2, possibly via the 5'-3' ssDNA translocase activity of HELB (PubMed:26774285). As cells approach S phase, DNA end resection is promoted by the nuclear export of HELB following phosphorylation (PubMed:26774285). Acts independently of TP53BP1 (PubMed:26774285). Unwinds duplex DNA with 5'-3' polarity. Has single-strand DNA-dependent ATPase and DNA helicase activities. Prefers ATP and dATP as substrates (PubMed:12181327). During S phase, may facilitate cellular recovery from replication stress (PubMed:22194613). |
Cellular Location | Nucleus. Cytoplasm. Chromosome. Note=Predominantly nuclear (PubMed:15146062). Phosphorylation at Ser-967 by CDK2 during the G1/S transition results in its nuclear export into the cytoplasm as cells approach and progress through S phase (PubMed:15146062). Following DNA damage, recruited to sites of double-strand breaks by the RPA complex (PubMed:26774285). Recruited to chromatin following DNA damage induced by UV irradiation, or camptothecin or hydroxyurea treatment (PubMed:22194613). |
Tissue Location | Highly expressed in testis and thymus and weakly in liver, spleen, kidney and brain. |
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Provided below are standard protocols that you may find useful for product applications.
References
Taneja,P., et al., (2002) J. Biol. Chem. 277 (43), 40853-40861Reconstitution and Storage:For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
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