|Application ||WB, IHC|
|Other Accession||NM_002388, NP_002379|
|Reactivity||Human, Mouse, Rat, Rabbit, Horse, Yeast, Bovine, Dog|
|Predicted||Human, Mouse, Rat, Chicken, Horse, Bovine|
|Alias Symbol||HCC5, MGC1157, P1-MCM3, P1.h, RLFB|
|Other Names||DNA replication licensing factor MCM3, 22.214.171.124, DNA polymerase alpha holoenzyme-associated protein P1, P1-MCM3, RLF subunit beta, p102, MCM3|
|Format||Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.|
|Reconstitution & Storage||Add 50 ul of distilled water. Final anti-MCM3 antibody concentration is 1 mg/ml in PBS buffer with 2% sucrose. For longer periods of storage, store at 20°C. Avoid repeat freeze-thaw cycles.|
|Precautions||MCM3 antibody - C-terminal region is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. Required for DNA replication and cell proliferation.|
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Provided below are standard protocols that you may find useful for product applications.
Lin,D.I., (2008) Proc. Natl. Acad. Sci. U.S.A. 105 (23), 8079-8084 Reconstitution and Storage:For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
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