ASCL2 antibody - middle region
Rabbit Polyclonal Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| WB |
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Primary Accession | O35885 |
Other Accession | NM_008554, NP_032580 |
Reactivity | Human, Mouse, Rat, Rabbit, Zebrafish, Bovine, Dog |
Predicted | Mouse, Rat, Pig, Chicken, Bovine |
Host | Rabbit |
Clonality | Polyclonal |
Calculated MW | 29kDa |
Gene ID | 17173 |
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Alias Symbol | Mash2, bHLHa45, 2410083I15Rik |
Other Names | Achaete-scute homolog 2, ASH-2, mASH-2, mASH2, Ascl2, Mash2 |
Format | Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose. |
Reconstitution & Storage | Add 100 ul of distilled water. Final anti-ASCL2 antibody concentration is 1 mg/ml in PBS buffer with 2% sucrose. For longer periods of storage, store at 20°C. Avoid repeat freeze-thaw cycles. |
Precautions | ASCL2 antibody - middle region is for research use only and not for use in diagnostic or therapeutic procedures. |
Name | Ascl2 |
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Synonyms | Mash2 |
Function | Transcription factor (PubMed:10611232, PubMed:29500235). Binds to E-box motifs 5'-CANNTG-3' in the regulatory elements of target genes, probably as a heterodimer with another basic helix-loop-helix (bHLH) protein such as the transcription factor TCF3 (PubMed:10611232, PubMed:29500235). May bind both open and closed chromatin, acting as a pioneer transcription factor to allow other factors to bind and activate lineage-specific genes (PubMed:29500235). Required during post-implantation development for the generation of some differentiated trophoblast cell types (PubMed:8090202). Transcriptional activity of ASCL2 may be antagonised in a subset of trophoblast cells by bHLH transcription factor HAND1, perhaps by competing for dimerization with other bHLH proteins (PubMed:10611232). Involved in differentiation and function of follicular T-helper (Tfh) cells, thereby playing a role in germinal center responses; probably modulates expression of genes involved in Tfh cell function, such as BCL6 (PubMed:24463518). May also act as a suppressor of Th1-, Th2- and Th17-cell differentiation (PubMed:24463518). Induces the formation of stem cells in intestinal crypts in vitro, synergistically activating transcription of target genes, such as SOX9, together with TCF4/beta-catenin (PubMed:25620640). May form a bistable transcriptional switch, controlling expression of its own gene together with Wnt/R-spondin signaling, and thereby maintaining stem cell characteristics (PubMed:25620640). Modulates expression of target genes, including perhaps down-regulating EGR1/Krox24 and chemokine CXCL10/Mob-1 and up-regulating CXCR4 and CDKN1C/p57kip2, in Schwann cells (By similarity). May play a role in reducing proliferation of Schwann cells, perhaps acting via modulation of expression of CDKN1C (By similarity). May be dispensable for blastocyst formation and later embryonic function (PubMed:8090202, PubMed:9622625). May be involved in the determination of neuronal precursors (By similarity). |
Cellular Location | Nucleus {ECO:0000250|UniProtKB:P19360}. |
Tissue Location | Expressed in follicular T-helper (Tfh) cells. |
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Provided below are standard protocols that you may find useful for product applications.
References
Georgiades,P. (2006) Development 133 (6), 1059-1068Reconstitution and Storage:For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
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