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> home > Products > Primary Antibodies > Antibody Collections > Zebrafish > MKL1 antibody - C-terminal region
MKL1 antibody - C-terminal region
Rabbit Polyclonal Antibody
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Application 
| WB |
|---|---|
| Primary Accession | Q8K4J6 |
| Other Accession | NM_153049, NP_694629 |
| Reactivity | Human, Mouse, Rat, Rabbit, Zebrafish, Pig, Horse, Bovine, Dog |
| Predicted | Human, Mouse, Rat, Rabbit, Pig, Chicken, Horse, Bovine, Dog |
| Host | Rabbit |
| Clonality | Polyclonal |
| Calculated MW | 106kDa |
| Gene ID | 223701 |
|---|---|
| Alias Symbol | Mal, AMKL, Bsac, Mrtf-A, AI852829, AW743281, AW821984 |
| Other Names | MKL/myocardin-like protein 1, Basic SAP coiled-coil transcription activator, Megakaryoblastic leukemia 1 protein homolog, Myocardin-related transcription factor A, MRTF-A, Mkl1, Bsac |
| Format | Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose. |
| Reconstitution & Storage | Add 100 ul of distilled water. Final anti-MKL1 antibody concentration is 1 mg/ml in PBS buffer with 2% sucrose. For longer periods of storage, store at 20°C. Avoid repeat freeze-thaw cycles. |
| Precautions | MKL1 antibody - C-terminal region is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | Mrtfa |
|---|---|
| Function | Transcription coactivator that associates with the serum response factor (SRF) transcription factor to control expression of genes regulating the cytoskeleton during development, morphogenesis and cell migration (PubMed:12019265, PubMed:12732141, PubMed:17588931, PubMed:19350017, PubMed:24732378). The SRF-MRTFA complex activity responds to Rho GTPase-induced changes in cellular globular actin (G- actin) concentration, thereby coupling cytoskeletal gene expression to cytoskeletal dynamics (PubMed:24732378). MRTFA binds G-actin via its RPEL repeats, regulating activity of the MRTFA-SRF complex (PubMed:12732141, PubMed:17588931). Activity is also regulated by filamentous actin (F-actin) in the nucleus (PubMed:23558171, PubMed:25759381). |
| Cellular Location | Cytoplasm. Nucleus Note=Subcellular location is tightly regulated by actin both in cytoplasm and nucleus: high levels of G-actin in the nucleus observed during serum deprivation lead to low levels of nuclear MRTFA, while reduced levels of nuclear G-actin result in accumulation of MRTFA in the nucleus (PubMed:17588931, PubMed:21673315). G-actin-binding in the cytoplasm inhibits nuclear import by masking the nuclear localization signal (NLS) (PubMed:17588931, PubMed:21673315). In contrast, binding to nuclear globular actin (G-actin) promotes nuclear export to the cytoplasm (PubMed:17588931). Nuclear localization is regulated by MICAL2, which mediates depolymerization of nuclear actin, which decreases nuclear G-actin pool, thereby promoting retention of MRTFA in the nucleus and subsequent formation of an active complex with SRF (By similarity). {ECO:0000250|UniProtKB:Q969V6, ECO:0000269|PubMed:17588931, ECO:0000269|PubMed:21673315} |
| Tissue Location | Expressed in heart, brain, spleen, lung, liver, muscle, kidney and testis. |
Research Areas
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Application Protocols
Provided below are standard protocols that you may find useful for product applications.
References
Sasazuki,T., et al., (2002) J. Biol. Chem. 277 (32), 28853-28860Reconstitution and Storage:For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
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$ 339.00
Cat# AI11238
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