Tppp antibody - C-terminal region
Rabbit Polyclonal Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| WB |
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Primary Accession | Q7TQD2 |
Other Accession | NM_182839, NP_878259 |
Reactivity | Human, Mouse, Rat, Rabbit, Pig, Horse, Bovine, Dog |
Predicted | Human, Mouse, Rat, Rabbit, Pig, Chicken, Bovine |
Host | Rabbit |
Clonality | Polyclonal |
Calculated MW | 24kDa |
Gene ID | 72948 |
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Alias Symbol | AI849835, 2900041A09Rik |
Other Names | Tubulin polymerization-promoting protein, TPPP, Tppp |
Format | Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose. |
Reconstitution & Storage | Add 100 ul of distilled water. Final anti-Tppp antibody concentration is 1 mg/ml in PBS buffer with 2% sucrose. For longer periods of storage, store at 20°C. Avoid repeat freeze-thaw cycles. |
Precautions | Tppp antibody - C-terminal region is for research use only and not for use in diagnostic or therapeutic procedures. |
Name | Tppp {ECO:0000303|PubMed:18028908, ECO:0000312|MGI:MGI:1920198} |
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Function | Regulator of microtubule dynamics that plays a key role in myelination by promoting elongation of the myelin sheath (PubMed:31522887). Acts as a microtubule nucleation factor in oligodendrocytes: specifically localizes to the postsynaptic Golgi apparatus region, also named Golgi outpost, and promotes microtubule nucleation, an important step for elongation of the myelin sheath (PubMed:31522887). Required for both uniform polarized growth of distal microtubules as well as directing the branching of proximal processes (PubMed:31522887). Shows magnesium-dependent GTPase activity; the role of the GTPase activity is unclear (By similarity). In addition to microtubule nucleation activity, also involved in microtubule bundling and stabilization of existing microtubules, thereby maintaining the integrity of the microtubule network (PubMed:18028908). Regulates microtubule dynamics by promoting tubulin acetylation: acts by inhibiting the tubulin deacetylase activity of HDAC6 (By similarity). Also regulates cell migration: phosphorylation by ROCK1 inhibits interaction with HDAC6, resulting in decreased acetylation of tubulin and increased cell motility (By similarity). Plays a role in cell proliferation by regulating the G1/S-phase transition (By similarity). Involved in astral microtubule organization and mitotic spindle orientation during early stage of mitosis; this process is regulated by phosphorylation by LIMK2 (By similarity). |
Cellular Location | Golgi outpost {ECO:0000250|UniProtKB:D3ZQL7}. Cytoplasm, cytoskeleton, microtubule organizing center {ECO:0000250|UniProtKB:D3ZQL7}. Cytoplasm, cytoskeleton. Nucleus Cytoplasm, cytoskeleton, spindle {ECO:0000250|UniProtKB:O94811} Note=Specifically localizes to the postsynaptic Golgi apparatus region, also named Golgi outpost, which shapes dendrite morphology by functioning as sites of acentrosomal microtubule nucleation (By similarity). Mainly localizes to the cytoskeleton (PubMed:18028908) Also found in the nucleus; however, nuclear localization is unclear and requires additional evidences (PubMed:18028908). Localizes to glial Lewy bodies in the brains of individuals with synucleinopathies. During mitosis, colocalizes with LIMK2 at the mitotic spindle (By similarity) {ECO:0000250|UniProtKB:D3ZQL7, ECO:0000250|UniProtKB:O94811, ECO:0000269|PubMed:18028908} |
Tissue Location | Widely expressed with higher expression in brain (at protein level). |
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Provided below are standard protocols that you may find useful for product applications.
References
Tirian,L., et al., (2003) Proc. Natl. Acad. Sci. U.S.A. 100 (24), 13976-13981Reconstitution and Storage:For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
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