ZNF598 antibody - middle region
Rabbit Polyclonal Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| WB |
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Primary Accession | Q86UK7 |
Other Accession | NM_178167, NP_835461 |
Reactivity | Human, Mouse, Rat, Horse, Dog |
Predicted | Horse, Dog |
Host | Rabbit |
Clonality | Polyclonal |
Calculated MW | 99kDa |
Gene ID | 90850 |
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Alias Symbol | DKFZp762F135, FLJ00086 |
Other Names | Zinc finger protein 598, ZNF598 |
Format | Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose. |
Reconstitution & Storage | Add 50 ul of distilled water. Final anti-ZNF598 antibody concentration is 1 mg/ml in PBS buffer with 2% sucrose. For longer periods of storage, store at 20°C. Avoid repeat freeze-thaw cycles. |
Precautions | ZNF598 antibody - middle region is for research use only and not for use in diagnostic or therapeutic procedures. |
Name | ZNF598 {ECO:0000303|PubMed:28132843, ECO:0000312|HGNC:HGNC:28079} |
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Function | E3 ubiquitin-protein ligase that plays a key role in the ribosome quality control (RQC), a pathway that takes place when a ribosome has stalled during translation, leading to degradation of nascent peptide chains (PubMed:28065601, PubMed:28132843, PubMed:28685749, PubMed:32579943, PubMed:32099016, PubMed:33581075). ZNF598 is activated when ribosomes are stalled within an mRNA following translation of prematurely polyadenylated mRNAs (PubMed:28065601, PubMed:28132843, PubMed:28685749). Acts as a ribosome collision sensor: specifically recognizes and binds collided di-ribosome, which arises when a trailing ribosome encounters a slower leading ribosome, leading to terminally arrest translation (PubMed:28065601, PubMed:28132843, PubMed:28685749, PubMed:30293783). Following binding to colliding ribosomes, mediates monoubiquitination of 40S ribosomal proteins RPS10/eS10 and RPS3/uS3, and 'Lys-63'-linked polyubiquitination of RPS20/uS10 (PubMed:28065601, PubMed:28132843, PubMed:28685749). Polyubiquitination of RPS20/uS10 promotes recruitment of the RQT (ribosome quality control trigger) complex, which drives the disassembly of stalled ribosomes, followed by degradation of nascent peptides (PubMed:32579943, PubMed:32099016, PubMed:36302773). E3 ubiquitin-protein ligase activity is dependent on the E2 ubiquitin- conjugating enzyme UBE2D3 (PubMed:28685749). Also acts as an adapter that recruits the 4EHP-GYF2 complex to mRNAs (PubMed:22751931, PubMed:32726578). Independently of its role in RQC, may also act as a negative regulator of interferon-stimulated gene (ISG) expression (PubMed:29719242). |
Cellular Location | Cytoplasm, cytosol. |
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Provided below are standard protocols that you may find useful for product applications.
References
Tao,W.A., (2005) Nat. Methods 2 (8), 591-598 Reconstitution and Storage:For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
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