KLHL3 antibody - N-terminal region
Rabbit Polyclonal Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| WB |
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Primary Accession | Q9UH77 |
Other Accession | NM_017415, NP_059111 |
Reactivity | Human, Rat, Rabbit, Bovine, Dog |
Predicted | Human, Rabbit, Bovine, Dog |
Host | Rabbit |
Clonality | Polyclonal |
Calculated MW | 65kDa |
Gene ID | 26249 |
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Alias Symbol | PHA2D |
Other Names | Kelch-like protein 3, KLHL3, KIAA1129 |
Format | Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose. |
Reconstitution & Storage | Add 100 ul of distilled water. Final anti-KLHL3 antibody concentration is 1 mg/ml in PBS buffer with 2% sucrose. For longer periods of storage, store at 20°C. Avoid repeat freeze-thaw cycles. |
Precautions | KLHL3 antibody - N-terminal region is for research use only and not for use in diagnostic or therapeutic procedures. |
Name | KLHL3 {ECO:0000303|PubMed:10843806, ECO:0000312|HGNC:HGNC:6354} |
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Function | Substrate-specific adapter of a BCR (BTB-CUL3-RBX1) E3 ubiquitin ligase complex that acts as a regulator of ion transport in the distal nephron (PubMed:14528312, PubMed:22406640, PubMed:23387299, PubMed:23453970, PubMed:23576762, PubMed:23665031, PubMed:25313067, PubMed:35093948). The BCR(KLHL3) complex acts by mediating ubiquitination and degradation of WNK1 and WNK4, two activators of Na- Cl cotransporter SLC12A3/NCC in distal convoluted tubule cells of kidney, thereby regulating NaCl reabsorption (PubMed:23387299, PubMed:23453970, PubMed:23576762, PubMed:23665031, PubMed:25313067, PubMed:35093948). The BCR(KLHL3) complex also mediates ubiquitination and degradation of WNK3 (PubMed:35179207). The BCR(KLHL3) complex also mediates ubiquitination of CLDN8, a tight-junction protein required for paracellular chloride transport in the kidney, leading to its degradation (By similarity). |
Cellular Location | Cytoplasm, cytosol. Cytoplasm, cytoskeleton |
Tissue Location | Widely expressed.. |
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Provided below are standard protocols that you may find useful for product applications.
References
Lai,F., et al., (2000) Genomics 66 (1), 65-75Reconstitution and Storage:For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
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