|Other Accession||NM_019852, NP_062826|
|Reactivity||Human, Mouse, Rat, Rabbit, Zebrafish, Pig, Goat, Horse, Yeast, Bovine, Dog|
|Predicted||Mouse, Rat, Rabbit, Pig, Bovine|
|Alias Symbol||M6A, MGC4336, MT-A70, Spo8, IME4|
|Other Names||N6-adenosine-methyltransferase 70 kDa subunit, MT-A70, 220.127.116.11, Methyltransferase-like protein 3, METTL3, MTA70|
|Format||Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.|
|Reconstitution & Storage||Add 50 ul of distilled water. Final anti-METTL3 antibody concentration is 1 mg/ml in PBS buffer with 2% sucrose. For longer periods of storage, store at 20°C. Avoid repeat freeze-thaw cycles.|
|Precautions||METTL3 antibody - middle region is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||The METTL3-METTL14 heterodimer forms a N6- methyltransferase complex that methylates adenosine residues of some RNAs and regulates the circadian clock, differentiation of embryonic stem cells and primary miRNA processing (PubMed:22575960, PubMed:24284625, PubMed:25719671, PubMed:25799998, PubMed:26321680, PubMed:26593424, PubMed:27373337, PubMed:27281194, PubMed:9409616). In the heterodimer formed with METTL14, METTL3 constitutes the catalytic core (PubMed:27373337, PubMed:27281194). N6-methyladenosine (m6A), which takes place at the 5'-[AG]GAC-3' consensus sites of some mRNAs, plays a role in the efficiency of mRNA splicing, processing, translation efficiency, editing and mRNA stability (PubMed:22575960, PubMed:24284625, PubMed:25719671, PubMed:25799998, PubMed:26321680, PubMed:26593424, PubMed:9409616). M6A regulates the length of the circadian clock: acts as an early pace-setter in the circadian loop by putting mRNA production on a fast-track for facilitating nuclear processing, thereby providing an early point of control in setting the dynamics of the feedback loop (By similarity). M6A also acts as a regulator of mRNA stability: in embryonic stem cells (ESCs), m6A methylation of mRNAs encoding key naive pluripotency-promoting transcripts results in transcript destabilization, promoting differentiation of ESCs (By similarity). M6A also takes place in other RNA molecules, such as primary miRNA (pri-miRNAs) (PubMed:25799998). METTL3 also mediates methylation of pri-miRNAs, marking them for recognition and processing by DGCR8 (PubMed:25799998). Acts as a positive regulator of mRNA translation independently of the methyltransferase activity: promotes translation by interacting with the translation initiation machinery in the cytoplasm (PubMed:27117702). Its overexpression in a number of cancer cells suggests that it may participate to cancer cell proliferation by promoting mRNA translation (PubMed:27117702).|
|Cellular Location||Nucleus. Nucleus speckle. Cytoplasm. Note=Colocalizes with speckles in interphase nuclei. Suggesting that it may be associated with nuclear pre-mRNA splicing components|
|Tissue Location||Widely expressed at low level. Expressed in spleen, thymus, prostate, testis, ovary, small intestine, colon and peripheral blood leukocytes.|
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Provided below are standard protocols that you may find useful for product applications.
Beausoleil,S.A., (2006) Nat. Biotechnol. 24 (10), 1285-1292 Reconstitution and Storage:For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
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