|Application ||WB, IHC|
|Other Accession||NM_001536, NP_001527|
|Reactivity||Human, Mouse, Rat, Rabbit, Zebrafish, Pig, Horse, Bovine, Dog|
|Predicted||Human, Mouse, Rabbit, Zebrafish, Pig, Bovine|
|Alias Symbol||ANM1, HCP1, IR1B4, HRMT1L2|
|Other Names||Protein arginine N-methyltransferase 1, 2.1.1.-, Histone-arginine N-methyltransferase PRMT1, 22.214.171.124, Interferon receptor 1-bound protein 4, PRMT1, HMT2, HRMT1L2, IR1B4|
|Format||Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.|
|Reconstitution & Storage||Add 100 ul of distilled water. Final anti-PRMT1 antibody concentration is 1 mg/ml in PBS buffer with 2% sucrose. For longer periods of storage, store at 20°C. Avoid repeat freeze-thaw cycles.|
|Precautions||PRMT1 antibody - middle region is for research use only and not for use in diagnostic or therapeutic procedures.|
|Synonyms||HMT2, HRMT1L2, IR1B4|
|Function||Arginine methyltransferase that methylates (mono and asymmetric dimethylation) the guanidino nitrogens of arginyl residues present in proteins such as ESR1, histone H2, H3 and H4, PIAS1, HNRNPA1, HNRNPD, NFATC2IP, SUPT5H, TAF15 and EWS. Constitutes the main enzyme that mediates monomethylation and asymmetric dimethylation of histone H4 'Arg-4' (H4R3me1 and H4R3me2a, respectively), a specific tag for epigenetic transcriptional activation. Together with dimethylated PIAS1, represses STAT1 transcriptional activity, in the late phase of interferon gamma (IFN-gamma) signaling. May be involved in the regulation of TAF15 transcriptional activity, act as an activator of estrogen receptor (ER)-mediated transactivation, play a key role in neurite outgrowth and act as a negative regulator of megakaryocytic differentiation, by modulating p38 MAPK pathway. Methylates FOXO1 and retains it in the nucleus increasing its transcriptional activity. Methylates CHTOP and this methylation is critical for its 5-hydroxymethylcytosine (5hmC)-binding activity (PubMed:25284789). Methylates H4R3 in genes involved in glioblastomagenesis in a CHTOP- and/or TET1-dependent manner (PubMed:25284789).|
|Cellular Location||Nucleus. Nucleus, nucleoplasm. Cytoplasm, cytosol. Note=Mostly found in the cytoplasm. Colocalizes with CHTOP within the nucleus. Low levels detected also in the chromatin fraction (By similarity).|
|Tissue Location||Widely expressed.|
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Provided below are standard protocols that you may find useful for product applications.
Adams,M.M., (2005) Cell Cycle 4 (12), 1854-1861Reconstitution and Storage:For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
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