|Application ||WB, IHC|
|Other Accession||NM_012433, NP_036565|
|Reactivity||Human, Mouse, Rat, Rabbit, Zebrafish, Pig, Goat, Horse, Bovine, Dog|
|Predicted||Human, Mouse, Rat, Rabbit, Zebrafish, Pig, Chicken, Bovine, Dog|
|Alias Symbol||PRP10, SAP155, SF3b155, MDS, Hsh155, PRPF10|
|Other Names||Splicing factor 3B subunit 1, Pre-mRNA-splicing factor SF3b 155 kDa subunit, SF3b155, Spliceosome-associated protein 155, SAP 155, SF3B1, SAP155|
|Format||Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.|
|Reconstitution & Storage||Add 100 ul of distilled water. Final anti-SF3B1 antibody concentration is 1 mg/ml in PBS buffer with 2% sucrose. For longer periods of storage, store at 20°C. Avoid repeat freeze-thaw cycles.|
|Precautions||SF3B1 antibody - N-terminal region is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Subunit of the splicing factor SF3B required for 'A' complex assembly formed by the stable binding of U2 snRNP to the branchpoint sequence (BPS) in pre-mRNA. Sequence independent binding of SF3A/SF3B complex upstream of the branch site is essential, it may anchor U2 snRNP to the pre-mRNA. May also be involved in the assembly of the 'E' complex. Belongs also to the minor U12-dependent spliceosome, which is involved in the splicing of rare class of nuclear pre-mRNA intron.|
|Cellular Location||Nucleus speckle. Note=During mitosis, transiently dispersed from the nuclear speckles to the cytoplasm|
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Provided below are standard protocols that you may find useful for product applications.
Beausoleil,S.A., (2004) Proc. Natl. Acad. Sci. U.S.A. 101 (33), 12130-12135 Reconstitution and Storage:For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
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