TRNT1 antibody - N-terminal region
Rabbit Polyclonal Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| WB, IHC |
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Primary Accession | Q96Q11 |
Other Accession | NM_016000, NP_057084 |
Reactivity | Human, Mouse, Rat, Rabbit, Zebrafish, Pig, Horse, Bovine, Dog |
Predicted | Human, Mouse, Rat, Pig, Chicken, Bovine |
Host | Rabbit |
Clonality | Polyclonal |
Calculated MW | 45kDa |
Gene ID | 51095 |
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Alias Symbol | CCA1, MtCCA, CGI-47 |
Other Names | CCA tRNA nucleotidyltransferase 1, mitochondrial, 2.7.7.72, Mitochondrial tRNA nucleotidyl transferase, CCA-adding, mt CCA-adding enzyme, mt tRNA CCA-diphosphorylase, mt tRNA CCA-pyrophosphorylase, mt tRNA adenylyltransferase, TRNT1 |
Format | Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose. |
Reconstitution & Storage | Add 100 ul of distilled water. Final anti-TRNT1 antibody concentration is 1 mg/ml in PBS buffer with 2% sucrose. For longer periods of storage, store at 20°C. Avoid repeat freeze-thaw cycles. |
Precautions | TRNT1 antibody - N-terminal region is for research use only and not for use in diagnostic or therapeutic procedures. |
Name | TRNT1 {ECO:0000303|PubMed:25193871, ECO:0000312|HGNC:HGNC:17341} |
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Function | Nucleotidyltransferase that catalyzes the addition and repair of the essential 3'-terminal CCA sequence in tRNAs, which is necessary for the attachment of amino acids to the 3' terminus of tRNA molecules, using CTP and ATP as substrates (PubMed:11504732, PubMed:31011209, PubMed:25193871, PubMed:25652405, PubMed:29454993, PubMed:34023389, PubMed:30959222, PubMed:25640237). tRNA 3'-terminal CCA addition is required both for tRNA processing and repair (PubMed:22076379, PubMed:25640237). Promotes tRNA repair and recycling downstream of the ribosome-associated quality control (RQC) pathway by mediating addition of the tRNA 3'-terminal CCA following cleavage by ANKZF1 and repair by ELAC1 (PubMed:31011209). Also involved in tRNA surveillance by mediating tandem CCA addition to generate a CCACCA at the 3' terminus of unstable tRNAs and tRNA-like transcripts (PubMed:22076379, PubMed:25640237). While stable tRNAs receive only 3'-terminal CCA, unstable tRNAs beginning with GG are marked with CCACCA and rapidly degraded (PubMed:22076379, PubMed:25640237). The structural flexibility of RNA controls the choice between CCA versus CCACCA addition: following the first CCA addition cycle, nucleotide-binding to the active site triggers a clockwise screw motion, producing torque on the RNA (PubMed:25640237). This ejects stable RNAs, whereas unstable RNAs are refolded while bound to the enzyme and subjected to a second CCA catalytic cycle (PubMed:25640237). |
Cellular Location | Mitochondrion. Cytoplasm. Nucleus |
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Provided below are standard protocols that you may find useful for product applications.
References
Augustin,M.A., (2003) J. Mol. Biol. 328 (5), 985-994Reconstitution and Storage:For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
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