|Other Accession||NM_016323, NP_057407|
|Reactivity||Human, Bovine, Dog|
|Predicted||Human, Bovine, Dog|
|Alias Symbol||CEB1, CEBP1|
|Other Names||E3 ISG15--protein ligase HERC5, 6.3.2.-, Cyclin-E-binding protein 1, HECT domain and RCC1-like domain-containing protein 5, HERC5, CEB1, CEBP1|
|Format||Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.|
|Reconstitution & Storage||Add 50 ul of distilled water. Final anti-HERC5 antibody concentration is 1 mg/ml in PBS buffer with 2% sucrose. For longer periods of storage, store at 20°C. Avoid repeat freeze-thaw cycles.|
|Precautions||HERC5 antibody - N-terminal region is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Major E3 ligase for ISG15 conjugation. Acts as a positive regulator of innate antiviral response in cells induced by interferon. Functions as part of the ISGylation machinery that recognizes target proteins in a broad and relatively non-specific manner. Catalyzes ISGylation of IRF3 which results in sustained activation, it attenuates IRF3-PIN1 interaction, which antagonizes IRF3 ubiquitination and degradation, and boosts the antiviral response. Catalyzes ISGylation of influenza A viral NS1 which attenuates virulence; ISGylated NS1 fails to form homodimers and thus to interact with its RNA targets. Catalyzes ISGylation of papillomavirus type 16 L1 protein which results in dominant- negative effect on virus infectivity. Physically associated with polyribosomes, broadly modifies newly synthesized proteins in a cotranslational manner. In an interferon-stimulated cell, newly translated viral proteins are primary targets of ISG15.|
|Cellular Location||Cytoplasm, perinuclear region. Note=Associated with the polyribosomes, probably via the 60S subunit|
|Tissue Location||Expressed in testis and to a lesser degree in brain, ovary and placenta. Found in most tissues at low levels|
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