|Other Accession||NM_194463, NP_919445|
|Reactivity||Human, Mouse, Rat, Rabbit, Pig, Horse, Bovine, Guinea Pig, Dog|
|Predicted||Human, Mouse, Rat, Dog|
|Alias Symbol||FLJ23516, GRAIL|
|Other Names||E3 ubiquitin-protein ligase RNF128, 6.3.2.-, Gene related to anergy in lymphocytes protein, GRAIL, RING finger protein 128, RNF128|
|Format||Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.|
|Reconstitution & Storage||Add 100 ul of distilled water. Final anti-RNF128 antibody concentration is 1 mg/ml in PBS buffer with 2% sucrose. For longer periods of storage, store at 20°C. Avoid repeat freeze-thaw cycles.|
|Precautions||RNF128 antibody - C-terminal region is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||E3 ubiquitin-protein ligase that catalyzes 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains formation. Functions as an inhibitor of cytokine gene transcription. Inhibits IL2 and IL4 transcription, thereby playing an important role in the induction of the anergic phenotype, a long-term stable state of T-lymphocyte unresponsiveness to antigenic stimulation associated with the blockade of interleukin production. Ubiquitinates ARPC5 with 'Lys- 48' linkages and COR1A with 'Lys-63' linkages leading to their degradation, down-regulation of these cytosleletal components results in impaired lamellipodium formation and reduced accumulation of F-actin at the immunological synapse. Functions in the patterning of the dorsal ectoderm; sensitizes ectoderm to respond to neural-inducing signals.|
|Cellular Location||Endomembrane system; Single- pass membrane protein. Cytoplasm, cytoskeleton Cytoplasm, perinuclear region. Note=Localized in an asymmetric perinuclear punctate manner. Localizes to the internal pool of the transferrin recycling endosomal pathway. Partially colocalized with the endoplasmic reticulum resident HSPA5, with Golgi resident STX5, and with the late endosomal GTPase RAB7A (By similarity).|
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