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>   home   >   Products   >   Primary Antibodies   >   Cell Biology   >   RNF128 antibody - C-terminal region   

RNF128 antibody - C-terminal region

Rabbit Polyclonal Antibody

     
  • WB - RNF128 antibody - C-terminal region AI12260

    WB Suggested Anti-RNF128 Antibody Titration: 1.25μg/ml
    Positive Control: HepG2 cell lysate
    RNF128 is supported by BioGPS gene expression data to be expressed in HepG2
  • WB - RNF128 antibody - C-terminal region AI12260
    Lanes: 1: CD4 T cell lysate
    from WT mice, 2: CD4 T cell lysate
    from WT mice treated with anti-CD3/CD28, 3: CD4 T cell lysate
    from WT mice treated with FK506, 4: CD4 T cell lysate
    from WT mice treated with anti-CD3/CD28 and FK506, 5: CD4 T cell lysate
    from TRPV1 KO mice, 6: CD4 T cell lysate
    from TRPV1 KO mice treated with anti-CD3/CD28, 7: CD4 T cell lysate
    from TRPV1 KO mice treated with FK506, 8: CD4 T cell lysate
    from TRPV1 KO mice treated with anti-CD3/CD28 and FK506.
    Primary Antibody Dilution: 1:1000
    Secondary Antibody: Anti-rabbit HRP
    Secondary Antibody Dilution: 1:10,000
    Gene Name: RNF128
    Submitted by: Christine Quinley, UCSD
  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB
Primary Accession Q8TEB7
Other Accession NM_194463, NP_919445
Reactivity Human, Mouse, Rat, Rabbit, Pig, Horse, Bovine, Guinea Pig, Dog
Predicted Human, Mouse, Rat, Dog
Host Rabbit
Clonality Polyclonal
Calculated MW 47kDa
Additional Information
Gene ID 79589
Alias Symbol FLJ23516, GRAIL
Other Names E3 ubiquitin-protein ligase RNF128, 6.3.2.-, Gene related to anergy in lymphocytes protein, GRAIL, RING finger protein 128, RNF128
Format Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Reconstitution & Storage Add 100 ul of distilled water. Final anti-RNF128 antibody concentration is 1 mg/ml in PBS buffer with 2% sucrose. For longer periods of storage, store at 20°C. Avoid repeat freeze-thaw cycles.
PrecautionsRNF128 antibody - C-terminal region is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name RNF128
Function E3 ubiquitin-protein ligase that catalyzes 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains formation. Functions as an inhibitor of cytokine gene transcription. Inhibits IL2 and IL4 transcription, thereby playing an important role in the induction of the anergic phenotype, a long-term stable state of T-lymphocyte unresponsiveness to antigenic stimulation associated with the blockade of interleukin production. Ubiquitinates ARPC5 with 'Lys- 48' linkages and COR1A with 'Lys-63' linkages leading to their degradation, down-regulation of these cytosleletal components results in impaired lamellipodium formation and reduced accumulation of F-actin at the immunological synapse. Functions in the patterning of the dorsal ectoderm; sensitizes ectoderm to respond to neural-inducing signals.
Cellular Location Endomembrane system; Single- pass membrane protein. Cytoplasm, cytoskeleton Cytoplasm, perinuclear region. Note=Localized in an asymmetric perinuclear punctate manner. Localizes to the internal pool of the transferrin recycling endosomal pathway. Partially colocalized with the endoplasmic reticulum resident HSPA5, with Golgi resident STX5, and with the late endosomal GTPase RAB7A (By similarity).
Research Areas
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$ 232.00
Cat# AI12260
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