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Hdac4 Antibody - C-terminal region

Rabbit Polyclonal Antibody

  • CHIP - Hdac4 Antibody - C-terminal region AI12303
    Chromatin Immunoprecipitation (ChIP) Using Hdac4 Antibody - C-terminal region and HCT116 Cells
  • WB - Hdac4 Antibody - C-terminal region AI12303

    WB Suggested Anti-Hdac4 Antibody Titration: 1.0 μg/ml
    Positive Control: Mouse Liver
  • WB - Hdac4 Antibody - C-terminal region AI12303
    Lanes: Lane 1: 40ug mouse brain, synaptosome lysate Lane 2: 40ug mouse brain, membrane fraction Lane 3: 40ug mouse brain, cytoplasm fraction Lane 4: 40ug mouse brain, nuclear fraction Lane 5: 40ug mouse brain, post synaptic density fraction Lane 6: 40ug mouse brain, synaptosome lysate Lane 7: 40ug mouse brain, membrane fraction Lane 8: 40ug mouse brain, cytoplasm fraction Lane 9: 40ug mouse brain, nuclear fraction
    Primary Antibody Dilution: 1:1000
    Secondary Antibody: Goat anti-rabbit HRP
    Secondary Antibody Dilution: 1:2000
    Gene Name: Hdac4
    Submitted by: Wen-Cheng Xiong, Georgia Health Sciences University
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q6NZM9
Other Accession NM_207225, NP_997108
Reactivity Human, Mouse, Rat, Zebrafish, Horse, Yeast, Bovine, Guinea Pig, Dog
Predicted Human, Mouse, Rat, Zebrafish, Pig, Horse, Yeast, Bovine, Guinea Pig, Dog
Host Rabbit
Clonality Polyclonal
Calculated MW 118kDa
Additional Information
Gene ID 208727
Alias Symbol 4932408F19Rik, AI047285
Other Names Histone deacetylase 4, HD4,, Hdac4
Format Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Reconstitution & Storage Add 50 ul of distilled water. Final anti-Hdac4 antibody concentration is 1 mg/ml in PBS buffer with 2% sucrose. For longer periods of storage, store at 20°C. Avoid repeat freeze-thaw cycles.
PrecautionsHdac4 Antibody - C-terminal region is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name Hdac4
Function Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Involved in muscle maturation via its interaction with the myocyte enhancer factors such as MEF2A, MEF2C and MEF2D (By similarity).
Cellular Location Nucleus. Cytoplasm. Note=Shuttles between the nucleus and the cytoplasm. Upon muscle cells differentiation, it accumulates in the nuclei of myotubes, suggesting a positive role of nuclear HDAC4 in muscle differentiation. The export to cytoplasm depends on the interaction with a 14-3-3 chaperone protein and is due to its phosphorylation at Ser-245, Ser-465 and Ser-629 by CaMK4 and SIK1. The nuclear localization probably depends on sumoylation (By similarity).
Research Areas
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$ 293.00
Cat# AI12303
(40 western blots)
Availability: 2-3 days
Bulk Size
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