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Fkrp antibody - C-terminal region

Rabbit Polyclonal Antibody

     
  • WB - Fkrp antibody - C-terminal region AI12520

    WB Suggested Anti-Fkrp Antibody Titration: 0.2-1 μg/ml
    ELISA Titer: 1:62500
    Positive Control: Mouse Heart
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB
Primary Accession Q8CG64
Other Accession NM_173430, NP_775606
Reactivity Human, Mouse, Rat, Rabbit, Zebrafish, Pig, Bovine, Guinea Pig, Dog
Predicted Human, Mouse, Rat, Rabbit, Zebrafish, Pig, Bovine
Host Rabbit
Clonality Polyclonal
Calculated MW 55kDa
Additional Information
Gene ID 243853
Alias Symbol A830029B19Rik, AI842067, AI847300, LGMD1I, MDC1C
Other Names Fukutin-related protein, 2.-.-.-, Fkrp
Format Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Reconstitution & Storage Add 50 ul of distilled water. Final anti-Fkrp antibody concentration is 1 mg/ml in PBS buffer with 2% sucrose. For longer periods of storage, store at 20°C. Avoid repeat freeze-thaw cycles.
PrecautionsFkrp antibody - C-terminal region is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name Fkrp {ECO:0000312|MGI:MGI:2447586}
Function Catalyzes the transfer of CDP-ribitol to ribitol 5-phosphate previously attached by FKTN/fukutin of to the phosphorylated O-mannosyl trisaccharide (N-acetylgalactosamine-beta-3-N-acetylglucosamine-beta-4- (phosphate-6-)mannose), a carbohydrate structure present in alpha- dystroglycan (DAG1) (PubMed:26923585). This constitutes the second step in the formation of the ribose 5-phosphate tandem repeat which links the phosphorylated O-mannosyl trisaccharide to the ligand binding moiety composed of repeats of 3-xylosyl-alpha-1,3-glucuronic acid-beta- 1 (PubMed:26923585).
Cellular Location Golgi apparatus membrane; Single-pass type II membrane protein. Secreted. Cell membrane, sarcolemma. Rough endoplasmic reticulum {ECO:0000250|UniProtKB:Q9H9S5}. Cytoplasm. Note=The N-terminal hydrophobic domain is cleaved after translocation to the Golgi apparatus and the protein is secreted (PubMed:19900540). Localization at the cell membrane may require the presence of dystroglycan (PubMed:17452335). At the Golgi apparatus localizes to the middle-to-trans-cisternae (PubMed:12471058, PubMed:17554798). Detected in rough endoplasmic reticulum in myocytes (By similarity). {ECO:0000250|UniProtKB:Q9H9S5, ECO:0000269|PubMed:12471058, ECO:0000269|PubMed:17452335, ECO:0000269|PubMed:17554798, ECO:0000269|PubMed:19900540}
Tissue Location Expressed in the retina, specifically in the inner segments of the photoreceptors, the outer plexiform layers, inner nuclear layers, and ganglion cell layers (at protein level) (PubMed:29416295). Expressed at highest levels in brain, lung, heart, kidney and liver (PubMed:12471058).
Research Areas
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$ 389.00
Cat# AI12520
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