|Application ||WB, IHC|
|Other Accession||NM_178009, NP_821077|
|Reactivity||Human, Mouse, Rat, Rabbit, Zebrafish, Pig, Horse, Bovine, Guinea Pig, Dog|
|Predicted||Human, Mouse, Rabbit, Zebrafish, Chicken, Horse, Bovine, Guinea Pig, Dog|
|Alias Symbol||DGKeta, DKFZp761I1510|
|Other Names||Diacylglycerol kinase eta, DAG kinase eta, 126.96.36.199, Diglyceride kinase eta, DGK-eta, DGKH|
|Format||Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.|
|Reconstitution & Storage||Add 50 ul of distilled water. Final anti-DGKH antibody concentration is 1 mg/ml in PBS buffer with 2% sucrose. For longer periods of storage, store at 20°C. Avoid repeat freeze-thaw cycles.|
|Precautions||DGKH antibody - middle region is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Phosphorylates diacylglycerol (DAG) to generate phosphatidic acid (PA). Plays a key role in promoting cell growth. Activates the Ras/B-Raf/C-Raf/MEK/ERK signaling pathway induced by EGF. Regulates the recruitment of RAF1 and BRAF from cytoplasm to membranes and their heterodimerization.|
|Cellular Location||Cytoplasm. Cell membrane Note=Translocated from the cytoplasm to endosomes in response to stress stimuli (PubMed:12810723). Isoform 2 is rapidly relocated back to the cytoplasm upon removal of stress stimuli, whereas isoform 1 exhibits sustained endosomal association (PubMed:12810723). Translocates from the cytoplasm to the cell membrane in the presence of active GTP-bound form of HRAS (PubMed:19710016)|
|Tissue Location||Isoform 2 is ubiquitously expressed. Isoform 1 is expressed only in testis, kidney and colon|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
firstname.lastname@example.org, and receive a free "I Love Antibodies" mug.
Provided below are standard protocols that you may find useful for product applications.
If you have used an Abgent product and would like to share how it has performed, please click on the "Submit Review" button and provide the requested information. Our staff will examine and post your review and contact you if needed.
If you have any additional inquiries please email technical services at email@example.com.