|Application ||WB, IHC|
|Other Accession||NM_178009, NP_821077|
|Reactivity||Human, Mouse, Rat, Rabbit, Zebrafish, Pig, Horse, Bovine, Guinea Pig, Dog|
|Predicted||Human, Mouse, Rabbit, Zebrafish, Chicken, Horse, Bovine, Guinea Pig, Dog|
|Alias Symbol||DGKeta, DKFZp761I1510|
|Other Names||Diacylglycerol kinase eta, DAG kinase eta, 184.108.40.206, Diglyceride kinase eta, DGK-eta, DGKH|
|Format||Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.|
|Reconstitution & Storage||Add 50 ul of distilled water. Final anti-DGKH antibody concentration is 1 mg/ml in PBS buffer with 2% sucrose. For longer periods of storage, store at 20°C. Avoid repeat freeze-thaw cycles.|
|Precautions||DGKH antibody - middle region is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Phosphorylates diacylglycerol (DAG) to generate phosphatidic acid (PA). Plays a key role in promoting cell growth. Activates the Ras/B-Raf/C-Raf/MEK/ERK signaling pathway induced by EGF. Regulates the recruitment of RAF1 and BRAF from cytoplasm to membranes and their heterodimerization.|
|Cellular Location||Cytoplasm. Cell membrane Note=Translocated from the cytoplasm to endosomes in response to stress stimuli (PubMed:12810723). Isoform 2 is rapidly relocated back to the cytoplasm upon removal of stress stimuli, whereas isoform 1 exhibits sustained endosomal association (PubMed:12810723). Translocates from the cytoplasm to the cell membrane in the presence of active GTP-bound form of HRAS (PubMed:19710016)|
|Tissue Location||Isoform 2 is ubiquitously expressed. Isoform 1 is expressed only in testis, kidney and colon|
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