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WBP11 antibody - N-terminal region

Rabbit Polyclonal Antibody

     
  • IHC - WBP11 antibody - N-terminal region AI12665
    Rabbit Anti-WBP11 Antibody
    Formalin Fixed Paraffin Embedded Tissue: Human Pineal Tissue Observed Staining: Cytoplasmic in cell bodies of pinealocytes and their processes
    Primary Antibody
    Concentration: 1:100 Other Working Concentrations: 1/600
    Secondary Antibody: Donkey anti-Rabbit-Cy3
    Secondary Antibody
    Concentration: 1:200
    Magnification: 20X
    Exposure Time: 0.5 - 2.0 sec
    detail
  • IP - WBP11 antibody - N-terminal region AI12665
    Amount and Sample Type : 500 ug mouse brain homogenate Amount of IP Antibody : 6 ug Primary Antibody : WBP11
    Primary Antibody Dilution : 1:500
    Secondary Antibody : Goat anti-rabbit Alexa-Fluor 594 Secondary Antibody Dilution : 1:5000
    Gene Name : WBP11 Submitted by : Dr. Yuzhi Chen, University of Arkansas for Medical Science
    detail
  • WB - WBP11 antibody - N-terminal region AI12665

    WB Suggested Anti-WBP11 Antibody Titration: 0.2-1 μg/ml
    ELISA Titer: 1:12500
    Positive Control: Hela cell lysate
    WBP11 is strongly supported by BioGPS gene expression data to be expressed in Human HeLa cells
    detail
  • WB - WBP11 antibody - N-terminal region AI12665
    Lanes: 1. Human NT-2 cells (60ug) lysate 2. Mouse WT brain extract (80ug)
    Primary Antibody Dilution: 2μg/ml
    Secondary Antibody: IRDye 800CW goat anti-rabbit from Li-COR Bioscience
    Secondary Antibody Dilution: 1: 20,000
    Gene Name: WBP11
    Submitted by: Dr. Yuzhi Chen, University of Arkansas for Medical Science
    detail
  • WB - WBP11 antibody - N-terminal region AI12665

    Host: Rabbit
    Target Name: WBP11
    Sample Tissue: Jurkat
    Antibody Dilution: 1.0μg/mlWBP11 is strongly supported by BioGPS gene expression data to be expressed in Jurkat
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC, IP
Primary Accession Q9Y2W2
Other Accession NM_016312, NP_057396
Reactivity Human, Mouse, Rat, Rabbit, Zebrafish, Pig, Horse, Bovine, Guinea Pig, Dog
Predicted Human, Mouse, Rat, Rabbit, Zebrafish, Horse, Guinea Pig, Dog
Host Rabbit
Clonality Polyclonal
Calculated MW 70kDa
Additional Information
Gene ID 51729
Alias Symbol DKFZp779M1063, NPWBP, SIPP1, WBP-11
Other Names WW domain-binding protein 11, WBP-11, Npw38-binding protein, NpwBP, SH3 domain-binding protein SNP70, Splicing factor that interacts with PQBP-1 and PP1, WBP11, NPWBP, SIPP1, SNP70
Format Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Reconstitution & Storage Add 50 ul of distilled water. Final anti-WBP11 antibody concentration is 1 mg/ml in PBS buffer with 2% sucrose. For longer periods of storage, store at 20°C. Avoid repeat freeze-thaw cycles.
PrecautionsWBP11 antibody - N-terminal region is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name WBP11 (HGNC:16461)
Synonyms NPWBP, SIPP1, SNP70
Function Activates pre-mRNA splicing. May inhibit PP1 phosphatase activity.
Cellular Location Nucleus. Cytoplasm. Note=Predominantly located in the nucleus with granular heterogeneous distribution. Excluded from nucleoli in interphase cells, distributed throughout cytoplasm in dividing cells. Colocalized with SC35 and U2B in the nucleus. In the cytoplasm, associates with the intermediate filament protein vimentin
Tissue Location Ubiquitous. Highly expressed in the heart, pancreas, kidney skeletal muscle, placenta and brain (at protein level). Weakly expressed in liver and lung
Research Areas
Citations (0)
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References

Olsen,J.V.,(2006)Cell127(3),635-648ReconstitutionandStorage:Forshorttermuse,storeat2-8Cupto1week.Forlongtermstorage,storeat-20Cinsmallaliquotstopreventfreeze-thawcycles.

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$ 389.00
Cat# AI12665
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