|Other Accession||NM_031145, NP_112407|
|Reactivity||Human, Mouse, Rat, Rabbit, Pig, Sheep, Horse, Bovine, Guinea Pig, Dog|
|Predicted||Human, Mouse, Rat, Rabbit, Pig, Sheep, Horse, Bovine, Guinea Pig, Dog|
|Alias Symbol||Cib, Sip2-28|
|Other Names||Calcium and integrin-binding protein 1, CIB, Calmyrin, DNA-PKcs-interacting protein, Kinase-interacting protein, KIP, Cib1, Cib, Kip, Prkdcip|
|Format||Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.|
|Reconstitution & Storage||Add 50 ul of distilled water. Final anti-Cib1 antibody concentration is 1 mg/ml in PBS buffer with 2% sucrose. For longer periods of storage, store at 20°C. Avoid repeat freeze-thaw cycles.|
|Precautions||Cib1 Antibody - N-terminal region is for research use only and not for use in diagnostic or therapeutic procedures.|
|Synonyms||Cib, Kip, Prkdcip|
|Function||Calcium-binding protein that plays a role in the regulation of numerous cellular processes, such as cell differentiation, cell division, cell proliferation, cell migration, thrombosis, angiogenesis, cardiac hypertrophy and apoptosis. Involved in bone marrow megakaryocyte differentiation by negatively regulating thrombopoietin-mediated signaling pathway. Participates in the endomitotic cell cycle of megakaryocyte, a form of mitosis in which both karyokinesis and cytokinesis are interrupted. Plays a role in integrin signaling by negatively regulating alpha-IIb/beta3 activation in thrombin- stimulated megakaryocytes preventing platelet aggregation. Up- regulates PTK2/FAK1 activity, and is also needed for the recruitment of PTK2/FAK1 to focal adhesions; it thus appears to play an important role in focal adhesion formation. Positively regulates cell migration on fibronectin in a CDC42-dependent manner, the effect being negatively regulated by PAK1. Functions as a negative regulator of stress activated MAP kinase (MAPK) signaling pathways. Down-regulates inositol 1,4,5-trisphosphate receptor-dependent calcium signaling. Involved in sphingosine kinase SPHK1 translocation to the plasma membrane in a N- myristoylation-dependent manner preventing TNF-alpha-induced apoptosis. Regulates serine/threonine-protein kinase PLK3 activity for proper completion of cell division progression. Plays a role in microtubule (MT) dynamics during neuronal development; disrupts the MT depolymerization activity of STMN2 attenuating NGF-induced neurite outgrowth and the MT reorganization at the edge of lamellipodia. Promotes cardiomyocyte hypertrophy via activation of the calcineurin/NFAT signaling pathway. Stimulates calcineurin PPP3R1 activity by mediating its anchoring to the sarcolemma. In ischemia-induced (pathological or adaptive) angiogenesis, stimulates endothelial cell proliferation, migration and microvessel formation by activating the PAK1 and ERK1/ERK2 signaling pathway. Promotes also cancer cell survival and proliferation. May regulate cell cycle and differentiation of spermatogenic germ cells, and/or differentiation of supporting Sertoli cells (By similarity).|
|Cellular Location||Membrane; Lipid-anchor. Cell membrane. Cell membrane, sarcolemma. Cytoplasm. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Nucleus Cytoplasm, perinuclear region. Cell projection, filopodium tip. Apical cell membrane. Cell projection, ruffle membrane. Cell projection, growth cone. Cell projection, lamellipodium Cytoplasm, cytoskeleton. Note=Colocalized with NBR1 to the perinuclear region. Colocalized with RAC3 in the perinuclear area and at the cell periphery. Colocalized with PAK1 within membrane ruffles during cell spreading upon readhesion to fibronectin. Redistributed to the cytoskeleton upon platelet aggregation. Translocates from the cytosol to the plasma membrane in a calcium-dependent manner. Colocalized with STMN2 to the leading edge of lamellipodia. Colocalizes with PLK3 at the centrosomes in ductal breast carcinoma cells. Colocalized with PPP3R1 at the cell membrane of cardiomyocytes in the hypertrophic heart (By similarity). Colocalized with STMN2 in the cell body, neurites and growth cones of neurons. Colocalizes with TAS1R2 in apical regions of taste receptor cells.|
|Tissue Location||Expressed in cardiomyocytes and neurons (at protein level). Expressed during early neural development|
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