Mbtd1 antibody - middle region
Rabbit Polyclonal Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| WB |
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Primary Accession | Q6P5G3 |
Other Accession | NM_134012, NP_598773 |
Reactivity | Human, Mouse, Rat, Rabbit, Pig, Horse, Bovine, Guinea Pig, Dog |
Predicted | Human, Mouse, Rabbit, Pig, Chicken, Horse, Bovine, Dog |
Host | Rabbit |
Clonality | Polyclonal |
Calculated MW | 71kDa |
Gene ID | 103537 |
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Alias Symbol | AA408199, AI194990, hemp |
Other Names | MBT domain-containing protein 1, Mbtd1 |
Format | Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose. |
Reconstitution & Storage | Add 50 ul of distilled water. Final anti-Mbtd1 antibody concentration is 1 mg/ml in PBS buffer with 2% sucrose. For longer periods of storage, store at 20°C. Avoid repeat freeze-thaw cycles. |
Precautions | Mbtd1 antibody - middle region is for research use only and not for use in diagnostic or therapeutic procedures. |
Name | Mbtd1 {ECO:0000312|MGI:MGI:2143977} |
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Function | Chromatin reader component of the NuA4 histone acetyltransferase complex, a multiprotein complex involved in transcriptional activation of select genes principally by acetylation of nucleosomal histones H4 and H2A. The NuA4 complex plays a direct role in repair of DNA double-strand breaks (DSBs) by promoting homologous recombination (HR). MBTD1 specifically recognizes and binds monomethylated and dimethylated 'Lys-20' on histone H4 (H4K20me1 and H4K20me2, respectively). In the NuA4 complex, MBTD1 promotes recruitment of the complex to H4K20me marks by competing with TP53BP1 for binding to H4K20me. Following recruitment to H4K20me at DNA breaks, the NuA4 complex catalyzes acetylation of 'Lys-15' on histone H2A (H2AK15), blocking the ubiquitination mark required for TP53BP1 localization at DNA breaks, thereby promoting homologous recombination (HR). |
Cellular Location | Nucleus {ECO:0000250|UniProtKB:Q05BQ5}. Chromosome {ECO:0000250|UniProtKB:Q05BQ5} |
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Provided below are standard protocols that you may find useful for product applications.
References
Church D.M.,et al.PLoS Biol. 7:E1000112-E1000112(2009).
Park J.,et al.Mol. Cell 50:919-930(2013).
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