|Other Accession||NM_178583, NP_848698|
|Reactivity||Human, Mouse, Rat, Rabbit, Pig, Horse, Bovine, Guinea Pig, Dog|
|Predicted||Human, Mouse, Rat, Rabbit, Pig, Chicken, Horse, Bovine, Guinea Pig, Dog|
|Alias Symbol||ALFY, KIAA0993, MGC16461, ZFYVE25|
|Other Names||WD repeat and FYVE domain-containing protein 3, Autophagy-linked FYVE protein, Alfy, WDFY3, KIAA0993|
|Format||Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.|
|Reconstitution & Storage||Add 50 ul of distilled water. Final anti-WDFY3 antibody concentration is 1 mg/ml in PBS buffer with 2% sucrose. For longer periods of storage, store at 20°C. Avoid repeat freeze-thaw cycles.|
|Precautions||WDFY3 antibody - C-terminal region is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Required for selective autophagy (aggrephagy) but not for autophagic degradation of bulk cytospol in response to starvation. Involved in the formation and degradation of cytoplasmic polyubiquitin-containing bodies (p62 bodies, ALIS/aggresome-like induced structures). May play a role as adaptor or scaffolding protein by promoting the association of the E3-like ligase ATG12-ATG5-ATG16L and LC3 to ubiquitinated target substrate. The association with GABARAP is required for its recruitment to LC3B-positive p62 bodies suggesting a role in targeting certain p62 structures for clearance. Involved in midbody ring degradation.|
|Cellular Location||Nucleus membrane. Cytoplasm. Nucleus, PML body. Membrane; Peripheral membrane protein; Cytoplasmic side Note=Colocalizes with autophagic structures in starved cells Shuttles between nucleus and cytoplasm; translocation from the nucleus to cytoplasmic polyubiquitin-containing bodies upon cellular stress is dependent on SQSTM1|
|Tissue Location||Ubiquitous. Expressed in osteoclast and their mononuclear precursors (at protein level)|
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Provided below are standard protocols that you may find useful for product applications.
Simonsen A.,et al.J. Cell Sci. 117:4239-4251(2004).
Hillier L.W.,et al.Nature 434:724-731(2005).
Nagase T.,et al.DNA Res. 6:63-70(1999).
Nakajima D.,et al.DNA Res. 9:99-106(2002).
Ota T.,et al.Nat. Genet. 36:40-45(2004).
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