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FLII antibody - middle region

Rabbit Polyclonal Antibody

  • IHC - FLII antibody - middle region AI13665
    FLII antibody - middle region (AI13665)
    Catalog Number: AI13665
    Formalin Fixed Paraffin Embedded Tissue: Human Bronchial Epithelial Tissue Observed Staining: Cytoplasm and membrane of bronchial epithelial tissue
    Primary Antibody
    Concentration: 1:600
    Secondary Antibody: Donkey anti-Rabbit-Cy3
    Secondary Antibody
    Concentration: 1:200
    Magnification: 20X
    Exposure Time: 0.5 - 2.0 sec
  • WB - FLII antibody - middle region AI13665

    WB Suggested Anti-FLII Antibody Titration: 0.2-1 μg/ml
    ELISA Titer: 1:62500
    Positive Control: OVCAR-3 cell lysate
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q13045
Other Accession NM_002018, NP_002009
Reactivity Human, Mouse, Rat, Horse, Bovine, Guinea Pig, Dog
Predicted Human, Mouse, Rat, Chicken, Horse, Bovine, Guinea Pig, Dog
Host Rabbit
Clonality Polyclonal
Calculated MW 145kDa
Additional Information
Gene ID 2314
Alias Symbol FLI, FLIL, Fli1, MGC39265
Other Names Protein flightless-1 homolog, FLII, FLIL
Format Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Reconstitution & Storage Add 50 ul of distilled water. Final anti-FLII antibody concentration is 1 mg/ml in PBS buffer with 2% sucrose. For longer periods of storage, store at 20°C. Avoid repeat freeze-thaw cycles.
PrecautionsFLII antibody - middle region is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Synonyms FLIL
Function May play a role as coactivator in transcriptional activation by hormone-activated nuclear receptors (NR) and acts in cooperation with NCOA2 and CARM1. Involved in estrogen hormone signaling. Involved in early embryonic development (By similarity). May play a role in regulation of cytoskeletal rearrangements involved in cytokinesis and cell migration, by inhibiting Rac1-dependent paxillin phosphorylation.
Cellular Location Nucleus. Cytoplasm, cytoskeleton. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cell junction, focal adhesion. Note=Colocalizes to actin-rich structures in blastocysts and, together with HRAS, RHOA and CDC42, in migrating fibroblasts. Localizes to centrosomes (By similarity)
Tissue Location Strongest expression in skeletal muscle with high expression also in the heart and lung
Research Areas
Citations (0)

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Campbell H.D.,et al.Genomics 42:46-54(1997).
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Zody M.C.,et al.Nature 440:1045-1049(2006).
Gevaert K.,et al.Nat. Biotechnol. 21:566-569(2003).
Campbell H.D.,et al.Proc. Natl. Acad. Sci. U.S.A. 90:11386-11390(1993).

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$ 293.00
Cat# AI13665
(40 western blots)
Availability: 2-3 days
Bulk Size
Seasonal Special on Bulk Order
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