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MED31 antibody - N-terminal region

Rabbit Polyclonal Antibody

     
  • CHIP - MED31 antibody - N-terminal region AI14009
    Quiescent human colon carcinoma HCT116 cultures were treated with 10% FBS for three time points (0, 15, 30min) or (0, 30, 60min) were used in Matrix-ChIP and real-time PCR assays at EGR1 gene (Exon1) and 15kb upstream site.
    detail
  • WB - MED31 antibody - N-terminal region AI14009

    WB Suggested Anti-MED31 Antibody Titration: 0.2-1 μg/ml
    ELISA Titer: 1:1562500
    Positive Control: Human Liver
    detail
  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, CHIP
Primary Accession Q9Y3C7
Other Accession NM_016055, NP_057144
Reactivity Human, Mouse, Rat, Rabbit, Horse, Bovine, Guinea Pig, Dog
Predicted Human, Mouse, Rat, Chicken, Bovine, Dog
Host Rabbit
Clonality Polyclonal
Calculated MW 16kDa
Additional Information
Gene ID 51003
Alias Symbol 3110004H13Rik, CGI-125, FLJ27436, FLJ36714, Soh1
Other Names Mediator of RNA polymerase II transcription subunit 31, Mediator complex subunit 31, Mediator complex subunit SOH1, hSOH1, MED31, SOH1
Format Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Reconstitution & Storage Add 50 ul of distilled water. Final anti-MED31 antibody concentration is 1 mg/ml in PBS buffer with 2% sucrose. For longer periods of storage, store at 20°C. Avoid repeat freeze-thaw cycles.
PrecautionsMED31 antibody - N-terminal region is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name MED31
Synonyms SOH1
Function Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene- specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.
Cellular Location Nucleus.
Citations (0)
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References

Lai C.-H.,et al.Genome Res. 10:703-713(2000).
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Mural R.J.,et al.Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
Gu W.,et al.Mol. Cell 3:97-108(1999).
Gu W.,et al.Mol. Cell 3:541-541(1999).

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$ 389.00
Cat# AI14009
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