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CENPN antibody - middle region

Rabbit Polyclonal Antibody

     
  • IF - CENPN antibody - middle region AI14084
    Sample Type : HeLa
    Primary Antibody Dilution: 4 μg/ml
    Secondary Antibody : Anti-rabbit Alexa 546
    Secondary Antibody Dilution: 2 μg/ml
    Gene Name : CENPN
  • IF - CENPN antibody - middle region AI14084
    Sample Type : Huh-7
    Primary Antibody Dilution: 4 μg/ml
    Secondary Antibody : Anti-rabbit Alexa 546
    Secondary Antibody Dilution: 2 μg/ml
    Gene Name : CENPN
  • IF - CENPN antibody - middle region AI14084
    Sample Type : MCF7
    Primary Antibody Dilution: 4 μg/ml
    Secondary Antibody : Anti-rabbit Alexa 546
    Secondary Antibody Dilution: 2 μg/ml
    Gene Name : CENPN
  • WB - CENPN antibody - middle region AI14084

    WB Suggested Anti-CENPN Antibody Titration: 0.2-1 μg/ml
    Positive Control: Jurkat cell lysate
    CENPN is supported by BioGPS gene expression data to be expressed in Jurkat
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IF
Primary Accession Q96H22
Other Accession NM_001100625, NP_001094095
Reactivity Human, Mouse, Rat, Rabbit, Bovine, Guinea Pig, Dog
Predicted Human, Mouse, Rat, Rabbit, Chicken, Bovine, Dog
Host Rabbit
Clonality Polyclonal
Calculated MW 41kDa
Additional Information
Gene ID 55839
Alias Symbol BM039, C16orf60, CENP-N, FLJ13607, FLJ22660
Other Names Centromere protein N, CENP-N, Interphase centromere complex protein 32, CENPN, C16orf60, ICEN32
Format Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Reconstitution & Storage Add 50 ul of distilled water. Final anti-CENPN antibody concentration is 1 mg/ml in PBS buffer with 2% sucrose. For longer periods of storage, store at 20°C. Avoid repeat freeze-thaw cycles.
PrecautionsCENPN antibody - middle region is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name CENPN
Synonyms C16orf60, ICEN32
Function Component of the CENPA-NAC (nucleosome-associated) complex, a complex that plays a central role in assembly of kinetochore proteins, mitotic progression and chromosome segregation. The CENPA-NAC complex recruits the CENPA-CAD (nucleosome distal) complex and may be involved in incorporation of newly synthesized CENPA into centromeres. CENPN is the first protein to bind specifically to CENPA nucleosomes and the direct binding of CENPA nucleosomes by CENPN is required for centromere assembly. Required for chromosome congression and efficiently align the chromosomes on a metaphase plate.
Cellular Location Nucleus. Chromosome, centromere, kinetochore. Note=Localizes exclusively in the kinetochore domain of centromeres. Kinetochore-bound levels decrease when cells enter mitosis and increase again when cells exit mitosis
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$ 293.00
Cat# AI14084
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