|Application ||WB, IF|
|Other Accession||NM_001100625, NP_001094095|
|Reactivity||Human, Mouse, Rat, Rabbit, Bovine, Guinea Pig, Dog|
|Predicted||Human, Mouse, Rat, Rabbit, Chicken, Bovine, Dog|
|Alias Symbol||BM039, C16orf60, CENP-N, FLJ13607, FLJ22660|
|Other Names||Centromere protein N, CENP-N, Interphase centromere complex protein 32, CENPN, C16orf60, ICEN32|
|Format||Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.|
|Reconstitution & Storage||Add 50 ul of distilled water. Final anti-CENPN antibody concentration is 1 mg/ml in PBS buffer with 2% sucrose. For longer periods of storage, store at 20°C. Avoid repeat freeze-thaw cycles.|
|Precautions||CENPN antibody - middle region is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Component of the CENPA-NAC (nucleosome-associated) complex, a complex that plays a central role in assembly of kinetochore proteins, mitotic progression and chromosome segregation. The CENPA-NAC complex recruits the CENPA-CAD (nucleosome distal) complex and may be involved in incorporation of newly synthesized CENPA into centromeres. CENPN is the first protein to bind specifically to CENPA nucleosomes and the direct binding of CENPA nucleosomes by CENPN is required for centromere assembly. Required for chromosome congression and efficiently align the chromosomes on a metaphase plate.|
|Cellular Location||Nucleus. Chromosome, centromere, kinetochore. Note=Localizes exclusively in the kinetochore domain of centromeres. Kinetochore-bound levels decrease when cells enter mitosis and increase again when cells exit mitosis|
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