UBE2O antibody - middle region
Rabbit Polyclonal Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| WB |
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Primary Accession | Q9C0C9 |
Other Accession | NM_022066, NP_071349 |
Reactivity | Human, Mouse, Rat, Rabbit, Horse, Bovine, Guinea Pig, Dog |
Predicted | Human, Mouse, Rat, Rabbit, Chicken, Horse, Bovine, Guinea Pig, Dog |
Host | Rabbit |
Clonality | Polyclonal |
Calculated MW | 141kDa |
Gene ID | 63893 |
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Alias Symbol | E2-230K, FLJ12878, KIAA1734 |
Other Names | E2/E3 hybrid ubiquitin-protein ligase UBE2O, 6.3.2.-, 6.3.2.19, Ubiquitin carrier protein O, Ubiquitin-conjugating enzyme E2 O, Ubiquitin-conjugating enzyme E2 of 230 kDa, Ubiquitin-conjugating enzyme E2-230K, Ubiquitin-protein ligase O, UBE2O, KIAA1734 |
Format | Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose. |
Reconstitution & Storage | Add 50 ul of distilled water. Final anti-UBE2O antibody concentration is 1 mg/ml in PBS buffer with 2% sucrose. For longer periods of storage, store at 20°C. Avoid repeat freeze-thaw cycles. |
Precautions | UBE2O antibody - middle region is for research use only and not for use in diagnostic or therapeutic procedures. |
Name | UBE2O |
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Synonyms | KIAA1734 |
Function | E2/E3 hybrid ubiquitin-protein ligase that displays both E2 and E3 ligase activities and mediates monoubiquitination of target proteins (PubMed:23455153, PubMed:24703950). Negatively regulates TRAF6-mediated NF-kappa-B activation independently of its E2 activity (PubMed:23381138). Acts as a positive regulator of BMP7 signaling by mediating monoubiquitination of SMAD6, thereby regulating adipogenesis (PubMed:23455153). Mediates monoubiquitination at different sites of the nuclear localization signal (NLS) of BAP1, leading to cytoplasmic retention of BAP1. Also able to monoubiquitinate the NLS of other chromatin-associated proteins, such as INO80 and CXXC1, affecting their subcellular location (PubMed:24703950). Acts as a regulator of retrograde transport by assisting the TRIM27:MAGEL2 E3 ubiquitin ligase complex to mediate 'Lys-63'-linked ubiquitination of WASHC1, leading to promote endosomal F-actin assembly (PubMed:23452853). |
Cellular Location | Cytoplasm. Nucleus. Note=Mainly localizes to the cytoplasm |
Tissue Location | Predominantly expressed in skeletal muscle and heart. |
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Provided below are standard protocols that you may find useful for product applications.
References
Nagase T.,et al.DNA Res. 7:347-355(2000).
Zody M.C.,et al.Nature 440:1045-1049(2006).
Mural R.J.,et al.Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
Bechtel S.,et al.BMC Genomics 8:399-399(2007).
Ota T.,et al.Nat. Genet. 36:40-45(2004).
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