|Application ||WB, IHC|
|Other Accession||NM_009360, NP_033386|
|Alias Symbol||AI661103, Hmgts, mtTFA, tsHMG|
|Other Names||Transcription factor A, mitochondrial, mtTFA, Testis-specific high mobility group protein, TS-HMG, Tfam, Hmgts|
|Format||Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.|
|Reconstitution & Storage||Add 50 ul of distilled water. Final anti-Tfam antibody concentration is 1 mg/ml in PBS buffer with 2% sucrose. For longer periods of storage, store at 20°C. Avoid repeat freeze-thaw cycles.|
|Precautions||Tfam antibody - middle region is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Isoform Mitochondrial binds to the mitochondrial light strand promoter and functions in mitochondrial transcription regulation. Required for accurate and efficient promoter recognition by the mitochondrial RNA polymerase. Promotes transcription initiation from the HSP1 and the light strand promoter by binding immediately upstream of transcriptional start sites. Is able to unwind DNA. Bends the mitochondrial light strand promoter DNA into a U-turn shape via its HMG boxes. Required for maintenance of normal levels of mitochondrial DNA. May play a role in organizing and compacting mitochondrial DNA. Isoform Nuclear may also function as a transcriptional activator or may have a structural role in the compaction of nuclear DNA during spermatogenesis.|
|Cellular Location||Isoform Mitochondrial: Mitochondrion. Mitochondrion matrix, mitochondrion nucleoid|
|Tissue Location||The mitochondrial isoform is widely expressed while the nuclear isoform is testis-specific|
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Provided below are standard protocols that you may find useful for product applications.
Larsson N.G.,et al.Nat. Genet. 13:296-302(1996).
Carninci P.,et al.Science 309:1559-1563(2005).
Cermakian N.,et al.Submitted (AUG-1996) to the EMBL/GenBank/DDBJ databases.
Boissonneault G.,et al.Mol. Cell. Biol. 13:4323-4330(1993).
Larsson N.G.,et al.Nat. Genet. 18:231-236(1998).
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