|Other Accession||NM_016794, NP_058074|
|Reactivity||Human, Mouse, Rat, Rabbit, Horse, Bovine, Guinea Pig, Dog|
|Predicted||Human, Mouse, Rat, Rabbit, Bovine, Guinea Pig, Dog|
|Alias Symbol||AU041171, Edb, endobrevin|
|Other Names||Vesicle-associated membrane protein 8, VAMP-8, Endobrevin, Edb, Vamp8|
|Format||Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.|
|Reconstitution & Storage||Add 50 ul of distilled water. Final anti-Vamp8 antibody concentration is 1 mg/ml in PBS buffer with 2% sucrose. For longer periods of storage, store at 20°C. Avoid repeat freeze-thaw cycles.|
|Precautions||Vamp8 antibody - middle region is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||SNAREs, Soluble N-ethylmaleimide-sensitive factor- attachment protein receptors, are essential proteins for fusion of cellular membranes. SNAREs localized on opposing membranes assemble to form a trans-SNARE complex, an extended, parallel four alpha-helical bundle that drives membrane fusion. VAMP8 is a SNARE involved in autophagy through the direct control of autophagosome membrane fusion with the lysososome membrane. Also required for dense-granule secretion in platelets. Plays also a role in regulated enzyme secretion in pancreatic acinar cells. Involved in the abscission of the midbody during cell division, which leads to completely separate daughter cells. Involved in the homotypic fusion of early and late endosomes.|
|Cellular Location||Lysosome membrane; Single-pass type IV membrane protein. Late endosome membrane; Single-pass type IV membrane protein Note=Perinuclear vesicular structures of the early and late endosomes, coated pits, and trans-Golgi. Sub-tight junctional domain in retinal pigment epithelium cells. Midbody region during cytokinesis. Lumenal oriented, apical membranes of nephric tubular cell. Cycles through the apical but not through the basolateral plasma membrane. Apical region of acinar cells; in zymogen granule membranes (By similarity).|
|Tissue Location||Expressed abundantly in the kidney, less in the liver, brain, kidney, heart, lung, pancreas and placenta|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
Wong S.H.,et al.Mol. Biol. Cell 9:1549-1563(1998).
Advani R.J.,et al.J. Biol. Chem. 273:10317-10324(1998).
Lu L.,et al.Submitted (MAR-2000) to the EMBL/GenBank/DDBJ databases.
Carninci P.,et al.Science 309:1559-1563(2005).
Wang C.-C.,et al.Dev. Cell 7:359-371(2004).
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