|Other Accession||NM_145973, NP_666085|
|Reactivity||Human, Mouse, Rat, Rabbit, Pig, Horse, Bovine, Dog|
|Predicted||Human, Mouse, Rat, Rabbit, Pig, Horse, Bovine, Dog|
|Other Names||RNA polymerase II elongation factor ELL3, Ell3|
|Format||Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.|
|Reconstitution & Storage||Add 50 ul of distilled water. Final anti-Ell3 antibody concentration is 1 mg/ml in PBS buffer with 2% sucrose. For longer periods of storage, store at 20°C. Avoid repeat freeze-thaw cycles.|
|Precautions||Ell3 Antibody - C-terminal region is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Enhancer-binding elongation factor that specifically binds enhancers in embryonic stem cells (ES cells), marks them, and is required for their future activation during stem cell specification. Elongation factor component of the super elongation complex (SEC), a complex required to increase the catalytic rate of RNA polymerase II transcription by suppressing transient pausing by the polymerase at multiple sites along the DNA. Component of the little elongation complex (LEC), a complex required to regulate small nuclear RNA (snRNA) gene transcription by RNA polymerase II and III. Does not only bind to enhancer regions of active genes, but also marks the enhancers that are in a poised or inactive state in ES cells and is required for establishing proper RNA polymerase II occupancy at developmentally regulated genes in a cohesin-dependent manner. Probably required for priming developmentally regulated genes for later recruitment of the super elongation complex (SEC), for transcriptional activation during differentiation. Required for recruitment of P- TEFb within SEC during differentiation. Probably preloaded on germ cell chromatin, suggesting that it may prime gene activation by marking enhancers as early as in the germ cells. Promoting epithelial-mesenchymal transition (EMT).|
|Tissue Location||Actively expressed in embryonic stem cells (ES cells), while it is weakly expressed in differentiated cells|
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Provided below are standard protocols that you may find useful for product applications.
Church D.M.,et al.PLoS Biol. 7:E1000112-E1000112(2009).
Lin C.,et al.Mol. Cell 37:429-437(2010).
Ahn H.J.,et al.PLoS ONE 7:E40293-E40293(2012).
Lin C.,et al.Cell 152:144-156(2013).
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