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>   home   >   Products   >   Primary Antibodies   >   TOP1MT Antibody - C-terminal region   

TOP1MT Antibody - C-terminal region

Rabbit Polyclonal Antibody

     
  • WB - TOP1MT Antibody - C-terminal region AI15353

    WB Suggested Anti-TOP1MT Antibody Titration: 1.0 μg/ml
    Positive Control: HepG2 Whole Cell
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB
Primary Accession Q969P6
Other Accession NM_052963, NP_443195
Reactivity Human, Mouse, Rat, Rabbit, Pig, Horse, Yeast, Bovine, Guinea Pig, Dog
Predicted Human, Mouse, Rat, Rabbit, Pig, Horse, Yeast, Bovine, Guinea Pig, Dog
Host Rabbit
Clonality Polyclonal
Calculated MW 70kDa
Additional Information
Gene ID 116447
Other Names DNA topoisomerase I, mitochondrial, TOP1mt, 5.99.1.2, TOP1MT
Format Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Reconstitution & Storage Add 50 ul of distilled water. Final anti-TOP1MT antibody concentration is 1 mg/ml in PBS buffer with 2% sucrose. For longer periods of storage, store at 20°C. Avoid repeat freeze-thaw cycles.
PrecautionsTOP1MT Antibody - C-terminal region is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name TOP1MT
Function Releases the supercoiling and torsional tension of DNA introduced during duplication of mitochondrial DNA by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(3'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 5'-OH DNA strand. The free DNA strand then rotates around the intact phosphodiester bond on the opposing strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 5'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone (By similarity).
Cellular Location Mitochondrion.
Tissue Location Ubiquitous; highest in skeletal muscle, heart, brain and fetal liver.
Citations (0)

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References

Zhang H.,et al.Proc. Natl. Acad. Sci. U.S.A. 98:10608-10613(2001).
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Nusbaum C.,et al.Nature 439:331-335(2006).

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$ 293.00
Cat# AI15353
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