|Other Accession||NM_052963, NP_443195|
|Reactivity||Human, Mouse, Rat, Rabbit, Pig, Horse, Yeast, Bovine, Guinea Pig, Dog|
|Predicted||Human, Mouse, Rat, Rabbit, Pig, Horse, Yeast, Bovine, Guinea Pig, Dog|
|Other Names||DNA topoisomerase I, mitochondrial, TOP1mt, 188.8.131.52, TOP1MT|
|Format||Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.|
|Reconstitution & Storage||Add 50 ul of distilled water. Final anti-TOP1MT antibody concentration is 1 mg/ml in PBS buffer with 2% sucrose. For longer periods of storage, store at 20°C. Avoid repeat freeze-thaw cycles.|
|Precautions||TOP1MT Antibody - C-terminal region is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Releases the supercoiling and torsional tension of DNA introduced during duplication of mitochondrial DNA by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(3'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 5'-OH DNA strand. The free DNA strand then rotates around the intact phosphodiester bond on the opposing strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 5'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone (By similarity).|
|Tissue Location||Ubiquitous; highest in skeletal muscle, heart, brain and fetal liver.|
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Provided below are standard protocols that you may find useful for product applications.
Zhang H.,et al.Proc. Natl. Acad. Sci. U.S.A. 98:10608-10613(2001).
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Nusbaum C.,et al.Nature 439:331-335(2006).
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