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>   home   >   Products   >   Primary Antibodies   >   Cell Biology   >   CDK8 antibody - C-terminal region   

CDK8 antibody - C-terminal region

Rabbit Polyclonal Antibody

     
  • WB - CDK8 antibody - C-terminal region AI16184

    WB Suggested Anti-CDK8 Antibody Titration: 1.25μg/ml
    ELISA Titer: 1:12500
    Positive Control: HepG2 cell lysate
  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB
Primary Accession P49336
Other Accession NM_001260, NP_001251
Reactivity Human, Mouse, Rabbit, Pig, Horse, Bovine, Dog
Predicted Human, Mouse, Rabbit, Pig, Chicken, Horse, Bovine, Dog
Host Rabbit
Clonality Polyclonal
Calculated MW 53kDa
Additional Information
Gene ID 1024
Alias Symbol K35
Other Names Cyclin-dependent kinase 8, 2.7.11.22, 2.7.11.23, Cell division protein kinase 8, Mediator complex subunit CDK8, Mediator of RNA polymerase II transcription subunit CDK8, Protein kinase K35, CDK8
Format Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Reconstitution & Storage Add 100 ul of distilled water. Final anti-CDK8 antibody concentration is 1 mg/ml in PBS buffer with 2% sucrose. For longer periods of storage, store at 20°C. Avoid repeat freeze-thaw cycles.
PrecautionsCDK8 antibody - C-terminal region is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name CDK8
Function Component of the Mediator complex, a coactivator involved in regulated gene transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. Phosphorylates the CTD (C-terminal domain) of the large subunit of RNA polymerase II (RNAp II), which may inhibit the formation of a transcription initiation complex. Phosphorylates CCNH leading to down-regulation of the TFIIH complex and transcriptional repression. Recruited through interaction with MAML1 to hyperphosphorylate the intracellular domain of NOTCH, leading to its degradation.
Cellular Location Nucleus.
Research Areas
Citations (0)

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Background

Component of the Mediator complex, a coactivator involved in regulated gene transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. Phosphorylates the CTD (C-terminal domain) of the large subunit of RNA polymerase II (RNAp II), which may inhibit the formation of a transcription initiation complex. Phosphorylates CCNH leading to down-regulation of the TFIIH complex and transcriptional repression. Recruited through interaction with MAML1 to hyperphosphorylate the intracellular domain of NOTCH, leading to its degradation.

References

Tassan J.-P.,et al.Proc. Natl. Acad. Sci. U.S.A. 92:8871-8875(1995).
Dunham A.,et al.Nature 428:522-528(2004).
Mural R.J.,et al.Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
Sun X.,et al.Mol. Cell 2:213-222(1998).
Gu W.,et al.Mol. Cell 3:97-108(1999).

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$ 232.00
Cat# AI16184
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