POU3F2 antibody - C-terminal region
Rabbit Polyclonal Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| WB |
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Primary Accession | P20265 |
Other Accession | NM_005604, NP_005595 |
Reactivity | Human, Rat, Pig, Bovine |
Predicted | Human, Rat, Pig, Bovine |
Host | Rabbit |
Clonality | Polyclonal |
Calculated MW | 47kDa |
Gene ID | 5454 |
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Alias Symbol | BRN2, OCT7, OTF7, OTF-7, POUF3, brn-2, oct-7 |
Other Names | POU domain, class 3, transcription factor 2, Brain-specific homeobox/POU domain protein 2, Brain-2, Brn-2, Nervous system-specific octamer-binding transcription factor N-Oct-3, Octamer-binding protein 7, Oct-7, Octamer-binding transcription factor 7, OTF-7, POU3F2, BRN2, OCT7, OTF7 |
Format | Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose. |
Reconstitution & Storage | Add 50 ul of distilled water. Final anti-POU3F2 antibody concentration is 1 mg/ml in PBS buffer with 2% sucrose. For longer periods of storage, store at 20°C. Avoid repeat freeze-thaw cycles. |
Precautions | POU3F2 antibody - C-terminal region is for research use only and not for use in diagnostic or therapeutic procedures. |
Name | POU3F2 |
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Synonyms | BRN2, OCT7, OTF7 |
Function | Transcription factor that plays a key role in neuronal differentiation (By similarity). Binds preferentially to the recognition sequence which consists of two distinct half-sites, ('GCAT') and ('TAAT'), separated by a non-conserved spacer region of 0, 2, or 3 nucleotides (By similarity). Acts as a transcriptional activator when binding cooperatively with SOX4, SOX11, or SOX12 to gene promoters (By similarity). The combination of three transcription factors, ASCL1, POU3F2/BRN2 and MYT1L, is sufficient to reprogram fibroblasts and other somatic cells into induced neuronal (iN) cells in vitro (By similarity). Acts downstream of ASCL1, accessing chromatin that has been opened by ASCL1, and promotes transcription of neuronal genes (By similarity). |
Cellular Location | Nucleus. |
Tissue Location | Expressed specifically in the neuroectodermal cell lineage |
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Provided below are standard protocols that you may find useful for product applications.
Background
Transcription factor that binds preferentially to the recognition sequence which consists of two distinct half-sites, ('GCAT') and ('TAAT'), separated by a nonconserved spacer region of 0, 2, or 3 nucleotides. Positively regulates the genes under the control of corticotropin-releasing hormone (CRH) and CRH II promoters (By similarity).
References
Schreiber E.,et al.Nucleic Acids Res. 21:253-258(1993).
Angus J.,et al.Oncogene 11:691-700(1995).
Mungall A.J.,et al.Nature 425:805-811(2003).
He X.,et al.Nature 340:35-42(1989).
Waragai M.,et al.Hum. Mol. Genet. 8:977-987(1999).
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