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>   home   >   Products   >   Primary Antibodies   >   Stem Cells   >   LEF1 antibody - N-terminal region   

LEF1 antibody - N-terminal region

Rabbit Polyclonal Antibody

     
  • WB - LEF1 antibody - N-terminal region AI16257

    WB Suggested Anti-LEF1 Antibody Titration: 0.2-1 μg/ml
    ELISA Titer: 1:312500
    Positive Control: Hela cell lysate
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB
Primary Accession Q9UJU2
Other Accession NM_016269, NP_057353
Reactivity Human, Mouse, Rat, Pig, Horse, Bovine, Guinea Pig, Dog
Predicted Human, Mouse, Rat, Pig, Horse, Bovine, Guinea Pig, Dog
Host Rabbit
Clonality Polyclonal
Calculated MW 44kDa
Additional Information
Gene ID 51176
Alias Symbol DKFZp586H0919, TCF1ALPHA, LEF-1, TCF10, TCF7L3
Other Names Lymphoid enhancer-binding factor 1, LEF-1, T cell-specific transcription factor 1-alpha, TCF1-alpha, LEF1
Format Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Reconstitution & Storage Add 50 ul of distilled water. Final anti-LEF1 antibody concentration is 1 mg/ml in PBS buffer with 2% sucrose. For longer periods of storage, store at 20°C. Avoid repeat freeze-thaw cycles.
PrecautionsLEF1 antibody - N-terminal region is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name LEF1
Function Participates in the Wnt signaling pathway. Activates transcription of target genes in the presence of CTNNB1 and EP300. May play a role in hair cell differentiation and follicle morphogenesis. TLE1, TLE2, TLE3 and TLE4 repress transactivation mediated by LEF1 and CTNNB1. Regulates T-cell receptor alpha enhancer function. Binds DNA in a sequence-specific manner. PIAG antagonizes both Wnt-dependent and Wnt-independent activation by LEF1 (By similarity). Isoform 3 lacks the CTNNB1 interaction domain and may be an antagonist for Wnt signaling. Isoform 5 transcriptionally activates the fibronectin promoter, binds to and represses transcription from the E-cadherin promoter in a CTNNB1- independent manner, and is involved in reducing cellular aggregation and increasing cell migration of pancreatic cancer cells. Isoform 1 transcriptionally activates MYC and CCND1 expression and enhances proliferation of pancreatic tumor cells.
Cellular Location Nucleus {ECO:0000255|PROSITE- ProRule:PRU00267}. Note=Found in nuclear bodies upon PIASG binding.
Tissue Location Detected in thymus. Not detected in normal colon, but highly expressed in colon cancer biopsies and colon cancer cell lines. Expressed in several pancreatic tumors and weakly expressed in normal pancreatic tissue. Isoforms 1 and 5 are detected in several pancreatic cell lines
Research Areas
Citations (0)

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Background

Participates in the Wnt signaling pathway. Activates transcription of target genes in the presence of CTNNB1 and EP300. May play a role in hair cell differentiation and follicle morphogenesis. TLE1, TLE2, TLE3 and TLE4 repress transactivation mediated by LEF1 and CTNNB1. Regulates T-cell receptor alpha enhancer function. Binds DNA in a sequence-specific manner. PIAG antagonizes both Wnt-dependent and Wnt-independent activation by LEF1 (By similarity). Isoform 3 lacks the CTNNB1 interaction domain and may be an antagonist for Wnt signaling. Isoform 5 transcriptionally activates the fibronectin promoter, binds to and represses transcription from the E-cadherin promoter in a CTNNB1- independent manner, and is involved in reducing cellular aggregation and increasing cell migration of pancreatic cancer cells. Isoform 1 transcriptionally activates MYC and CCND1 expression and enhances proliferation of pancreatic tumor cells.

References

Waterman M.L.,et al.Genes Dev. 5:656-669(1991).
Hovanes K.,et al.Nucleic Acids Res. 28:1994-2003(2000).
Jesse S.,et al.Int. J. Cancer 126:1109-1120(2010).
Kobielak A.,et al.Submitted (AUG-2000) to the EMBL/GenBank/DDBJ databases.
Ota T.,et al.Nat. Genet. 36:40-45(2004).

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$ 293.00
Cat# AI16257
Size:
Quantity:
(40 western blots)
Availability: 2-3 days
Bulk Size
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