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> home > Products > Primary Antibodies > Antibody Collections > Positive regulation of viral transcription > GTF2B antibody - N-terminal region
GTF2B antibody - N-terminal region
Rabbit Polyclonal Antibody
- SPECIFICATION
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- BACKGROUND
Application 
| WB |
|---|---|
| Primary Accession | Q00403 |
| Other Accession | NM_001514, NP_001505 |
| Reactivity | Human, Mouse, Rabbit, Pig, Horse, Guinea Pig, Dog |
| Predicted | Human, Mouse, Rabbit, Pig, Chicken, Horse, Guinea Pig, Dog |
| Host | Rabbit |
| Clonality | Polyclonal |
| Calculated MW | 35kDa |
| Gene ID | 2959 |
|---|---|
| Alias Symbol | TF2B, TFIIB |
| Other Names | Transcription initiation factor IIB, General transcription factor TFIIB, S300-II, GTF2B, TF2B, TFIIB |
| Format | Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose. |
| Reconstitution & Storage | Add 50 ul of distilled water. Final anti-GTF2B antibody concentration is 1 mg/ml in PBS buffer with 2% sucrose. For longer periods of storage, store at 20°C. Avoid repeat freeze-thaw cycles. |
| Precautions | GTF2B antibody - N-terminal region is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | GTF2B |
|---|---|
| Synonyms | TF2B, TFIIB |
| Function | General transcription factor that plays a role in transcription initiation by RNA polymerase II (Pol II). Involved in the pre-initiation complex (PIC) formation and Pol II recruitment at promoter DNA (PubMed:1876184, PubMed:1946368, PubMed:1517211, PubMed:3818643, PubMed:3029109, PubMed:8413225, PubMed:8515820, PubMed:8516311, PubMed:8516312, PubMed:7601352, PubMed:9420329, PubMed:12931194, PubMed:27193682). Together with the TATA box-bound TBP forms the core initiation complex and provides a bridge between TBP and the Pol II-TFIIF complex (PubMed:8504927, PubMed:8413225, PubMed:8515820, PubMed:8516311, PubMed:8516312). Released from the PIC early following the onset of transcription during the initiation and elongation transition and reassociates with TBP during the next transcription cycle (PubMed:7601352). Associates with chromatin to core promoter-specific regions (PubMed:12931194, PubMed:24441171). Binds to two distinct DNA core promoter consensus sequence elements in a TBP- independent manner; these IIB-recognition elements (BREs) are localized immediately upstream (BREu), 5'-[GC][GC][GA]CGCC-3', and downstream (BREd), 5'-[GA]T[TGA][TG][GT][TG][TG]-3', of the TATA box element (PubMed:9420329, PubMed:16230532, PubMed:7675079, PubMed:10619841). Modulates transcription start site selection (PubMed:10318856). Exhibits also autoacetyltransferase activity that contributes to the activated transcription (PubMed:12931194). |
| Cellular Location | Nucleus. Chromosome. Note=Non-acetylated form colocalizes with DNA in the G0/1, S and G2 phases of the cell cycle, but not during mitosis (PubMed:24441171). Acetylated form colocalizes at transcriptionally silent mitotic chromatids during mitosis at metaphase, anaphase, and telophase phases of the cell cycle (PubMed:24441171). |
| Tissue Location | Expressed in the inner cell mass forming the embryoblast (PubMed:24441171). Not detected in cells from the outer thin layer trophoblast (at protein level) (PubMed:24441171) |
Research Areas
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Application Protocols
Provided below are standard protocols that you may find useful for product applications.
Background
General factor that plays a major role in the activation of eukaryotic genes transcribed by RNA polymerase II.
References
Ha I.,et al.Nature 352:689-695(1991).
Malik S.,et al.Proc. Natl. Acad. Sci. U.S.A. 88:9553-9557(1991).
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Goshima N.,et al.Nat. Methods 5:1011-1017(2008).
Gregory S.G.,et al.Nature 441:315-321(2006).
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$ 389.00
Cat# AI16268
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