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Manufactured incorporating RabMAb® technology under Epitomics US patents, No 5675,063 and 7,429,487, owned by Abcam
YWHAB Antibody (C-term)Rabbit Monoclonal Antibody

Country
United States
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Ordering Information
Catalog # Size Availability Price  
AJ1001a 100µl 2-3 days $ 315.00 Add to cart
  • Specification
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YWHAB Antibody (C-term) - Product info

ApplicationWB, IHC
  • Applications Legend:
  • WB=Western Blotting
  • IP=Immunoprecipitation
  • IHC-P=Immunohistochemistry (Paraffin)
  • IF-IC=Immunofluorescence (Immunocytochemistry)
  • FC=Flow Cytometry
  • DB=Dot Blot
Primary AccessionP31946
ReactivityHuman, Mouse, Rat
Clone NamesY62
Calculated MW28082 Da
Gene ID 7529
Other Names
YWHAB, 14-3-3 protein beta/alpha, N-terminally processed, Protein 1054
Target/Specificity
A synthetic peptide corresponding to C-terminal residues of human 14-3-3 b/a was used as immunogen.
Dilution
WB~~1:1000~10000
IHC~~1:500
Format
50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Storage
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions
YWHAB Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.

YWHAB Antibody (C-term) - Protein Information

Name YWHAB
Function
Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. Negative regulator of osteogenesis. Blocks the nuclear translocation of the phosphorylated form (by AKT1) of SRPK2 and antagonizes its stimulatory effect on cyclin D1 expression resulting in blockage of neuronal apoptosis elicited by SRPK2.
Cellular Location
Cytoplasm. Melanosome. Note=Identified by mass spectrometry in melanosome fractions from stage I to stage IV

YWHAB Antibody (C-term) - Related products

AP8155a: YWHAB Antibody (N-term)

AP8155b: YWHAB Antibody (C-term)

BP8155a: YWHAB (14-3-3 protein beta/alpha) Antibody (N-term) Blocking Peptide

BP8155b: YWHAB (14-3-3 protein beta/alpha) Antibody (C-term) Blocking Peptide

AJ1001b: YWHAB Antibody (N-term)

AN1079: Phospho-Ser58 14-3-3 Protein Antibody

AN1075: 14-3-3 Protein Antibody

YWHAB Antibody (C-term) - Application data

  • A. Western blot analysis on Hela cell lysate using anti-14-3-3 b/a (C-term) RabMAb (Cat. #AJ1001a), dilution 1:1,000.

  • B. Immunohistochemical analysis of paraffin-embedded breast tissue anti-14-3-3 b/a (C-term) RabMAb (Cat. #AJ1001a).

YWHAB Antibody (C-term) - Research Areas

AntibodiesGene OntologyBiological ProcessApoptosisCell DeathSignal TransductionCellular ProcessPositive Regulation Of Biological ProcessPositive Regulation Of Cellular ProcessRegulation Of Cell DeathRegulation Of Programmed Cell DeathRegulation Of ApoptosisNegative Regulation Of Cellular ProcessNegative Regulation Of Biological ProcessRegulation Of Phosphate Metabolic ProcessRegulation Of Phosphorus Metabolic ProcessIntracellular Signaling CascadeRegulation Of Protein Metabolic ProcessRegulation Of Protein Modification ProcessRegulation Of Cellular Protein Metabolic ProcessRegulation Of Molecular FunctionPositive Regulation Of Molecular FunctionRegulation Of Biological QualityPositive Regulation Of Cell DeathPositive Regulation Of ApoptosisPositive Regulation Of Programmed Cell DeathRegulation Of Catalytic ActivityPositive Regulation Of Catalytic ActivityNegative Regulation Of Metabolic ProcessRegulation Of LocalizationRegulation Of TransportDeathProgrammed Cell DeathNegative Regulation Of Cellular Metabolic ProcessRegulation Of Metabolic ProcessMacromolecular Complex AssemblyProtein OligomerizationCellular Protein LocalizationCellular Macromolecule LocalizationCellular Component OrganizationMacromolecular Complex Subunit OrganizationNegative Regulation Of Macromolecule Metabolic ProcessCellular Component AssemblyCellular Component BiogenesisIntracellular Protein TransportRegulation Of Cellular Metabolic ProcessProtein Complex AssemblyProtein Complex BiogenesisNegative Regulation Of Protein Metabolic ProcessNegative Regulation Of Cellular Protein Metabolic ProcessNegative Regulation Of TransportIntracellular TransportNegative Regulation Of Phosphate Metabolic ProcessNegative Regulation Of Phosphorus Metabolic ProcessBiological RegulationRegulation Of Macromolecule Metabolic ProcessSmall GTPase Mediated Signal TransductionNegative Regulation Of Protein Modification ProcessEstablishment Of Localization In CellNegative Regulation Of Nitrogen Compound Metabolic ProcessRegulation Of Primary Metabolic ProcessProtein TargetingRegulation Of Biological ProcessNegative Regulation Of Nucleobase, Nucleoside, Nucleotide And Nucleic Acid Metabolic ProcessCellular LocalizationNegative Regulation Of Cellular Biosynthetic ProcessProtein HeterooligomerizationNegative Regulation Of Biosynthetic ProcessRegulation Of Establishment Of Protein LocalizationProtein TransportRegulation Of Cellular ProcessEstablishment Of Protein LocalizationNegative Regulation Of Gene ExpressionNegative Regulation Of TranscriptionNegative Regulation Of Macromolecule Biosynthetic ProcessRegulation Of Protein TransportRegulation Of Protein LocalizationMacromolecule LocalizationRas Protein Signal TransductionProtein LocalizationCellular CompartmentCytoplasmNucleusIntracellular PartIntracellularCytoplasmic PartMembrane-bounded OrganelleIntracellular Membrane-bounded OrganelleMembrane-enclosed LumenOrganelle LumenCytosolOrganelleIntracellular OrganelleIntracellular Organelle LumenOrganelle PartIntracellular Organelle PartNuclear LumenNuclear PartNucleoplasmPigment GranuleMelanosomePerinuclear Region Of CytoplasmVesicleNucleoplasm PartMembrane-bounded VesicleCytoplasmic VesicleCytoplasmic Membrane-bounded VesicleMacromolecular ComplexProtein ComplexMolecular FunctionCatalytic ActivityTranscription Cofactor ActivityTranscription Regulator ActivityProtein BindingEnzyme BindingOxidoreductase ActivityTranscription Factor BindingCarboxylic Acid BindingMonooxygenase ActivityAmino Acid BindingHistone Deacetylase BindingProtein Complex BindingAmine BindingProtein Domain Specific BindingProtein C-terminus BindingPathwaysKEGGCell CycleNeurotrophin Signaling PathwayOocyte MeiosisPantherP53 PathwayParkinson DiseaseFGF Signaling PathwayEGF Receptor Signaling PathwayPI3 Kinase PathwayDiseaseSystemic Lupus ErythematosusTissueSkinPlateletBrainColon CarcinomaKeratinocyte

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Provided below are standard protocols that you may find useful for product applications.

BACKGROUND

The 14-3-3 proteins are a family of proteins involved in the regulation of apoptosis, mitogenic signaling and cell-cycle checkpoints (1). The 14-3-3 proteins are thought to be key regulators of signal transduction events mediated through their binding to serine-phosphorylated proteins (2,3). Through binding Bad, 14-3-3 prevents apoptosis by sequestering Bad to the cytosol (3).

REFERENCES

1. Fu, H., et al. 14-3-3 proteins: structure, function, and regulation. Ann. Rev. Pharmacol. Toxicol. 40: 617
2. Muslin, A.J. et al. Interaction of 14-3-3 with Signaling Proteins Is Mediated by the Recognition of Phosphoserine. Cell 84: 889
3. Zha, J. et al. Serine Phosphorylation of Death Agonist BAD in Response to Survival Factor Results in Binding to 14-3-3 Not BCL-XL. Cell 87: 619