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YWHAB Antibody (C-term)

Rabbit Monoclonal Antibody

Manufactured incorporating RabMAb® technology under Epitomics US patents, No 5675,063 and 7,429,487, owned by Abcam
Country
United States
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Ordering Information
Catalog # SizeAvailability Price  
AJ1001a 100µl 2-3 days
$ 315.00email & fax$ 299.25online
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YWHAB Antibody (C-term) - Product info

Application
  • Applications Legend:
  • WB=Western Blotting
  • IP=Immunoprecipitation
  • IHC-P=Immunohistochemistry (Paraffin)
  • IF-IC=Immunofluorescence (Immunocytochemistry)
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, IHC
Primary AccessionP31946
ReactivityHuman, Mouse, Rat
Clone NamesY62
Calculated MW28082 Da
Gene ID 7529
Other Names
YWHAB, 14-3-3 protein beta/alpha, N-terminally processed, Protein 1054
Target/Specificity
A synthetic peptide corresponding to C-terminal residues of human 14-3-3 b/a was used as immunogen.
Dilution
WB~~1:1000~10000
IHC~~1:500
Format
50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Storage
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions
YWHAB Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.

YWHAB Antibody (C-term) - Protein Information

Name YWHAB
Function
Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. Negative regulator of osteogenesis. Blocks the nuclear translocation of the phosphorylated form (by AKT1) of SRPK2 and antagonizes its stimulatory effect on cyclin D1 expression resulting in blockage of neuronal apoptosis elicited by SRPK2.
Cellular Location
Cytoplasm. Melanosome. Note=Identified by mass spectrometry in melanosome fractions from stage I to stage IV

YWHAB Antibody (C-term) - Related products

AP8155a: YWHAB Antibody (N-term)

AP8155b: YWHAB Antibody (C-term)

BP8155a: YWHAB (14-3-3 protein beta/alpha) Antibody (N-term) Blocking Peptide

BP8155b: YWHAB (14-3-3 protein beta/alpha) Antibody (C-term) Blocking Peptide

AJ1001b: YWHAB Antibody (N-term)

AN1079: Phospho-Ser58 14-3-3 Protein Antibody

AN1075: 14-3-3 Protein Antibody

YWHAB Antibody (C-term) - Research Areas

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Provided below are standard protocols that you may find useful for product applications.

BACKGROUND

The 14-3-3 proteins are a family of proteins involved in the regulation of apoptosis, mitogenic signaling and cell-cycle checkpoints (1). The 14-3-3 proteins are thought to be key regulators of signal transduction events mediated through their binding to serine-phosphorylated proteins (2,3). Through binding Bad, 14-3-3 prevents apoptosis by sequestering Bad to the cytosol (3).

REFERENCES

1. Fu, H., et al. 14-3-3 proteins: structure, function, and regulation. Ann. Rev. Pharmacol. Toxicol. 40: 617
2. Muslin, A.J. et al. Interaction of 14-3-3 with Signaling Proteins Is Mediated by the Recognition of Phosphoserine. Cell 84: 889
3. Zha, J. et al. Serine Phosphorylation of Death Agonist BAD in Response to Survival Factor Results in Binding to 14-3-3 Not BCL-XL. Cell 87: 619