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4E-BP1/2/3 Antibody Phospho (pT45)Rabbit Monoclonal Antibody
| Country | United States
Ordering Information
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| Catalog # | Size | Availability | Price | |
| AJ1004f | 100ul 400 ul | 2-3 days | $ 355.00 | DISCONTINED INQUIRE CLICK INQUIRE Add to cart |
- Specification
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4E-BP1/2/3 Antibody Phospho (pT45) - Product info | |
| Application | WB, IHC
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| Primary Accession | Q13541 |
| Reactivity | Human, Mouse |
| Clone Names | EPR2169Y |
| Calculated MW | 12580 Da |
| Gene ID 1978 | |
| Other Names EIF4EBP1, Eukaryotic translation initiation factor 4E-binding protein 1, Phosphorylated heat- and acid-stable protein regulated by insulin 1 | |
| Target/Specificity A phospho specific peptide corresponding to residues surrounding threonine 45 was used as an immunogen. This antibody detects 4E-BP1/2/3 phosophorylated at threonine 45. | |
| Dilution WB~~1:500~1000 IHC~~1:50 | |
| Format 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. | |
| Storage Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. | |
| Precautions 4E-BP1/2/3 Antibody Phospho (pT45) is for research use only and not for use in diagnostic or therapeutic procedures. | |
4E-BP1/2/3 Antibody Phospho (pT45) - Protein Information | |
| Name EIF4EBP1 | |
| Function Regulates eIF4E activity by preventing its assembly into the eIF4F complex. Mediates the regulation of protein translation by hormones, growth factors and other stimuli that signal through the MAP kinase and mTORC1 pathways | |
4E-BP1/2/3 Antibody Phospho (pT45) - Related products
AP12627c: EIF4EBP1 Antibody (Center)
AP1981a: EIF4EBP1 Antibody (S100)
AP1981b: EIF4EBP1 Antibody (T36)
AP1981c: EIF4EBP1 Antibody (T69)
AP1981d: EIF4EBP1 Antibody(S111)
AP3353a: Phospho-EIF4EBP1-pS100 Antibody
AP3417a: Phospho-EIF4EBP1-pT36 Antibody
AP3473a: Phospho-EIF4EBP1-S111 Antibody
RI11733: EIF4EBP1 predesign siRNA
LY10995a: EIF4EBP1 Over-expression Lysate
BP1981a: EIF4EBP1 Antibody (S100) Blocking Peptide
BP1981b: EIF4EBP1 Antibody (T36) Blocking Peptide
BP1981c: EIF4EBP1 Antibody (T69) Blocking Peptide
BP3417a: Phospho-EIF4EBP1-pT36 Antibody Blocking Peptide
BP3473a: Phospho-EIF4EBP1-S111 Antibody Blocking Peptide
AJ1004d: 4E-BP1 Antibody Phospho (pT37)
AJ1004e: 4E-BP1 Antibody Phospho (pT70)
AJ1004f: 4E-BP1/2/3 Antibody Phospho (pT45)
4E-BP1/2/3 Antibody Phospho (pT45) - Application data
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A. Western blot analysis on 293T cell lysates using anti-Phospho-4E-Bp1/2/3 (pT45) RabMAb (Cat. #AJ1004f), 1:10,000 dilution. Cells were either (A) untreated (B) treated with FBS.
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B. Immunohistochemical analysis of paraffin-embedded human breast carcinoma using anti-4E-BPE1 RabMAb (Cat. #AJ1004f).
4E-BP1/2/3 Antibody Phospho (pT45) - Research Areas
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BACKGROUND
4E-BP1 (eIF4E-binding protein) also known as PHAS, is a 10-12 kDa acidic protein that compete with eIF4G for binding of eiF4E to the mRNA 5 cap structure (1). Binding of the 4E-BPs to eIF4E is reversible and is dependent on the phosphorylation status of 4E-BP. Non-phosphorylated 4E-BP1 will bind strongly to eiF4E while, the phosphorylated form will no (2)t. Akt, TOR, MAP kinase, S6 kinase, and Cdc2 are known kinases capable of inactivating 4E-BP1 binding to eIF4E by phosphorylating either threonines 35, 45, 69 or serine 64. Although, not all phosphorylation events equally block the 4EBP1-eIF4E interaction (3-4)
REFERENCES
1. Pause, A., et al. 1994. Nature 371: 762-767
2. Gingras, A.-C., et al. Genes & Dev. 12: 502-513, 1998
3. Iritani, BM et al. (1999) Proc. Natl. Acad. Sci. U. S. A. 96, 13180
4. Trumpp, A. et al. Nature 414, 768
