|Application ||WB, IHC|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||75592 Da|
|Other Names||ATP-binding cassette sub-family G member 1, ATP-binding cassette transporter 8, White protein homolog, ABCG1, ABC8, WHT1|
|Target/Specificity||A synthetic peptide corresponding to residues on human ABCG1 was used as an immunogen.|
|Format||50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||ABCG1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Transporter involved in macrophage lipid homeostasis. Is an active component of the macrophage lipid export complex. Could also be involved in intracellular lipid transport processes. The role in cellular lipid homeostasis may not be limited to macrophages.|
|Cellular Location||Endoplasmic reticulum membrane; Multi-pass membrane protein. Golgi apparatus membrane; Multi-pass membrane protein. Note=Predominantly localized in the intracellular compartments mainly associated with the endoplasmic reticulum (ER) and Golgi membranes|
|Tissue Location||Expressed in several tissues. Expressed in macrophages; expression is increased in macrophages from patients with Tangier disease.|
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Provided below are standard protocols that you may find useful for product applications.
ATP-binding cassette (ABC) transporter ABCG1 (ABC8) is induced in monocyte-derived macrophages during cholesterol influx mediated by acetylated low-density lipoprotein. Conversely, lipid efflux in cholesterol-laden macrophages, mediated by the cholesterol acceptor HDL(3), suppresses the expression of ABCG1. Immunocytochemical and flow cytometric analyses revealed that ABCG1 is expressed on the cell surface and in intracellular compartments of cholesterol-laden macrophages. Results demonstrate a regulator function for ABCG1 in cholesterol and phospholipid transport define a biologic activity for ABC transporters in macrophages (1). ABC transporters belonging to the ABCG (White) subfamily (ABCG1, ABCG5, and ABCG8) have been shown to be critically involved in the regulation of lipid-trafficking mechanisms in macrophages, hepatocytes, and intestinal mucosa cells (2) Studies show that treatment with Rhodobacter sphaeroiders LPS, an LPS antagonist, or PD169316, a specific p38 MAP kinase inhibitor, prevented the induction of ABCA1 by LPS. Therefore, this suggests that both transport of LPS from the plasma membrane to an intracellular site and activation of p38 MAP kinase are involved in the LPS-mediated induction of ABCA1 (3).
1. Klucken J, et al. Proc Natl Acad Sci 97(2):817-22, 2000
2. Kaplan R, et al. J Lipid Res 43(6):952-9, 2002.
3. Schmitz G, et al. J Lipid Res 42(10):1513-20, 2001
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