|Application ||WB, IHC, IF|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||46588 Da|
|Other Names||Medium-chain specific acyl-CoA dehydrogenase, mitochondrial, MCAD, ACADM|
|Target/Specificity||A synthetic peptide corresponding to residues in human ACADM was used as an immunogen.|
|Format||50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||ACADM Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||This enzyme is specific for acyl chain lengths of 4 to 16.|
|Cellular Location||Mitochondrion matrix.|
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Provided below are standard protocols that you may find useful for product applications.
Medium-chain specific acyl-CoA dehydrogenase (ACADM) is a homotetramer enzyme of the mitochondrial flavoenzyme family that catalyzes the initial reaction in medium chain fatty acid beta-oxidation (1, 2). ACADM is specific for acyl chain lengths of 4 to 16 (3). It utilizes the electron transfer flavoprotein (ETF) as an electron acceptor that transfers the electrons to the main mitochondrial respiratory chain via ETF-ubiquinone oxidoreductase (3). Defects in the ACADM gene cause medium-chain acyl-CoA dehydrogenase deficiency (MCADD), a metabolic disorder characterized by fasting hypoglycemia (3). Mutation analysis can diagnose MCADD, which is currently screened in newborns, since its monogene may be modified by genetic variations (4).
1. Zhang et al. Biochemistry 31:81-89, 1992. 2. Battaile et al. Elsevier Science B.V., 1998. 3. The UniProt Consortium, The Universal Protein Resource (UniProt), Nucleic Acids Res. 37:D169-D174(2009). 4. Gregersen et al. Hum Mutat.18(3):169-89, 2001.
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