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ACADM Antibody

Rabbit Monoclonal IgG

  • WB - ACADM Antibody AJ1007a
    Western blot analysis on (A) human heart, (B) fetal liver, (C) HeLa, (D) HepG2, and (E) K562 cell lysates using anti-ACADM RabMAb (Cat. #AJ1007a).
  • IHC - ACADM Antibody AJ1007a
    Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-ACADM RabMAb (Cat. #AJ1007a).
  • IF - ACADM Antibody AJ1007a
    Immunofluorescent staining of HeLa cells using anti-ACADM RabMAb (Cat. #AJ1007a).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P11310
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Monoclonal
Clone Names EPR3708
Calculated MW 46588 Da
Gene ID 34
Other Names Medium-chain specific acyl-CoA dehydrogenase, mitochondrial, MCAD, ACADM
Target/Specificity A synthetic peptide corresponding to residues in human ACADM was used as an immunogen.
Dilution WB~~1:1000~10000
Format 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsACADM Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Function This enzyme is specific for acyl chain lengths of 4 to 16.
Cellular Location Mitochondrion matrix.
Research Areas
Citations (0)

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Medium-chain specific acyl-CoA dehydrogenase (ACADM) is a homotetramer enzyme of the mitochondrial flavoenzyme family that catalyzes the initial reaction in medium chain fatty acid beta-oxidation (1, 2). ACADM is specific for acyl chain lengths of 4 to 16 (3). It utilizes the electron transfer flavoprotein (ETF) as an electron acceptor that transfers the electrons to the main mitochondrial respiratory chain via ETF-ubiquinone oxidoreductase (3). Defects in the ACADM gene cause medium-chain acyl-CoA dehydrogenase deficiency (MCADD), a metabolic disorder characterized by fasting hypoglycemia (3). Mutation analysis can diagnose MCADD, which is currently screened in newborns, since its monogene may be modified by genetic variations (4).


1. Zhang et al. Biochemistry 31:81-89, 1992. 2. Battaile et al. Elsevier Science B.V., 1998. 3. The UniProt Consortium, The Universal Protein Resource (UniProt), Nucleic Acids Res. 37:D169-D174(2009). 4. Gregersen et al. Hum Mutat.18(3):169-89, 2001.

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Cat# AJ1007a
(40 western blots)
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