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ACAT AntibodyRabbit Polyclonal IgG Affinity Purified
| Country | United States
Ordering Information
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| Catalog # | Size | Availability | Price | |
| AJ1008a | 100ul 400 ul | 2-3 days | $ 315.00 | DISCONTINED INQUIRE CLICK INQUIRE Add to cart |
- Specification
- Citiations : 0
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- Backgrounds
ACAT Antibody - Product info | |
| Application | WB, IHC
|
| Primary Accession | P23141 |
| Reactivity | Human, Mouse, Rat |
| Clone Names | Polyclonal |
| Calculated MW | 62521 Da |
| Gene ID 1066 | |
| Other Names CES1, CES2, SES1, Liver carboxylesterase 1, Egasyn;Retinyl ester hydrolase | |
| Target/Specificity A synthetic peptide corresponding to residues in human ACAT was used as immunogen. | |
| Dilution WB~~1:500~1000 IHC~~1:100~1:250 | |
| Format 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. | |
| Storage Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. | |
| Precautions ACAT Antibody is for research use only and not for use in diagnostic or therapeutic procedures. | |
ACAT Antibody - Protein Information | |
| Name CES1 | |
| Synonyms CES2, SES1 | |
| Function Involved in the detoxification of xenobiotics and in the activation of ester and amide prodrugs. Hydrolyzes aromatic and aliphatic esters, but has no catalytic activity toward amides or a fatty acyl-CoA ester. Hydrolyzes the methyl ester group of cocaine to form benzoylecgonine. Catalyzes the transesterification of cocaine to form cocaethylene. Displays fatty acid ethyl ester synthase activity, catalyzing the ethyl esterification of oleic acid to ethyloleate | |
| Cellular Location Endoplasmic reticulum lumen. | |
| Tissue Location Expressed predominantly in liver with lower levels in heart and lung | |
ACAT Antibody - Related products
AP9927b: CES1 Antibody (C-term)
BP9927b: CES1 Antibody (C-term) Blocking Peptide
ACAT Antibody - Application data
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A. Western blot analysis on fetal liver using anti-ACAT RabPAb (Cat. #AJ1008a), dilution 1:500.
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B. Immunohistochemical analysis of paraffin-embedded human liver using anti-ACAT RabPAb (Cat. #AJ1008a).
ACAT Antibody - Research Areas
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BACKGROUND
Acyl-CoA:cholesterol acyltransferase (ACAT) is an integral membrane enzyme that belongs to the type-B carboxylesterase/lipase family. ACAT catalyzes the formation of cholesteryl esters from cholesterol and long-chain fatty-acyl-coenzyme A (1). ACAT also function as a regulator of intracellular cholesterol homeostasis at a single cell level. Two isoforms of ACAT, ACAT1 and ACAT2, are expressed in the liver and intestine. However, ACAT1 is only found in macrophages and SMCs (2). The accumulation of cholesteryl esters produced by ACAT in macrophages can lead to foam cell formation, an indication of early stage atherosclerosis. ACAT activity has been linked to Alzheimer's Disease, a condition caused by excess amount of amyloid beta-peptide (Abeta). Atherogenic lipoproteins activate ACAT activity, where active ACAT can modulate the level of Abeta (3).
REFERENCES
1. Buhman KF et al. Biochim Biophys Acta 1529(1-3):142-54, 2000
