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ACAT AntibodyRabbit Monoclonal Antibody
| Country | United States
Ordering Information
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| Catalog # | Size | Availability | Price | |
| AJ1008b | 100ul 400 ul | 2-3 days | $ 315.00 | DISCONTINED INQUIRE CLICK INQUIRE Add to cart |
- Specification
- Citiations : 0
- Reviews
- Protocols
- Backgrounds
ACAT Antibody - Product info | |
| Application | WB
|
| Primary Accession | P23141 |
| Reactivity | Human, Mouse, Rat |
| Clone Names | EP1375Y |
| Calculated MW | 62521 Da |
| Gene ID 1066 | |
| Other Names CES1, CES2, SES1, Liver carboxylesterase 1, Egasyn;Retinyl ester hydrolase | |
| Target/Specificity A synthetic peptide corresponding to residues on the C-terminus of human ACAT was used as an immunogen. | |
| Dilution WB~~1:25000 | |
| Format 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. | |
| Storage Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. | |
| Precautions ACAT Antibody is for research use only and not for use in diagnostic or therapeutic procedures. | |
ACAT Antibody - Protein Information | |
| Name CES1 | |
| Synonyms CES2, SES1 | |
| Function Involved in the detoxification of xenobiotics and in the activation of ester and amide prodrugs. Hydrolyzes aromatic and aliphatic esters, but has no catalytic activity toward amides or a fatty acyl-CoA ester. Hydrolyzes the methyl ester group of cocaine to form benzoylecgonine. Catalyzes the transesterification of cocaine to form cocaethylene. Displays fatty acid ethyl ester synthase activity, catalyzing the ethyl esterification of oleic acid to ethyloleate | |
| Cellular Location Endoplasmic reticulum lumen. | |
| Tissue Location Expressed predominantly in liver with lower levels in heart and lung | |
ACAT Antibody - Related products
AP9927b: CES1 Antibody (C-term)
BP9927b: CES1 Antibody (C-term) Blocking Peptide
ACAT Antibody - Application data
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A. Western blot analysis on (A) U937 (B) human small intestine (C) human liver lysate using anti-ACAT RabMAb (Cat. #AJ1008b), dilution 1:50,000.
ACAT Antibody - Research Areas
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Provided below are standard protocols that you may find useful for product applications.
BACKGROUND
Triacylglycerol hydrolase is a microsomal enzyme that hydrolyzes stored cytoplasmic triacylglycerol in the liver and participates in the lipolysis/re-esterification cycle during the assembly of very-low-density lipoproteins. Expression of human TGH in Escherichia coli yields a protein without enzymatic activity, which suggests that posttranslational processing is necessary for the catalytic activity (1). ACAT esterifies free cholesterol and stores cholesteryl esters in lipid droplets. Macrophage ACAT1 deficiency results in increased atherosclerotic lesion area in hyperlipidemic mice via disrupted cholesterol efflux, increased lipoprotein uptake, accumulation of intracellular vesicles, and accelerated apoptosis (2). Data show that macrophage ACAT1 influences the efflux of both cellular and lipoprotein-derived cholesterol and propose a pathway for the pro-atherogenic transformation of ACAT1(-/-) macrophages (3).
REFERENCES
1. Alam M, et al. Biochemistry 41(21):6679-87, 2002.
2. Dove DE, et al. Atherosclerosis 186(2):267-74, 2006.
3. Sone DE, et al. Arterioscler Thromb Vasc Biol 25(1): 128-34, 2005.
