|Application ||WB, IHC|
|Calculated MW||149715 Da|
|Other Names||Angiotensin-converting enzyme, ACE, 321-, Dipeptidyl carboxypeptidase I, Kininase II, CD143, Angiotensin-converting enzyme, soluble form, ACE, DCP, DCP1|
|Target/Specificity||A synthetic peptide corresponding to residues on the C-terminus of human ACE was used as an immunogen.|
|Format||50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||ACE Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Converts angiotensin I to angiotensin II by release of the terminal His-Leu, this results in an increase of the vasoconstrictor activity of angiotensin. Also able to inactivate bradykinin, a potent vasodilator. Has also a glycosidase activity which releases GPI-anchored proteins from the membrane by cleaving the mannose linkage in the GPI moiety.|
|Cellular Location||Angiotensin-converting enzyme, soluble form: Secreted|
|Tissue Location||Ubiquitously expressed, with highest levels in lung, kidney, heart, gastrointestinal system and prostate. Isoform Testis-specific is expressed in spermatocytes and adult testis|
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Provided below are standard protocols that you may find useful for product applications.
Angiotensin-converting enzyme (ACE) is a zinc-containing dipeptidyl carboxypeptidase anchored in the plasma membrane with a carboxy-terminal hydrophobic domain (1, 2). ACE has a critical role in cardiovascular function because it catalyses the conversion of angiotensin I to the physiologically active vasopressor octapeptide angiotensin II, which controls fluid-electrolyte balance and systemic blood pressure (3, 4). Inhibitors of ACE are a first line of therapy for hypertension, heart failure, myocardial infarction and diabetic nephropathy (4). ACE also plays a critical role in the circulating or endocrine renin-angiotensin system (RAS) as well as the local regulation that exists in tissues such as the myocardium and skeletal muscle (5).
1. Ehlers MR, et al. Proc Natl Acad Sci U S A. 86(20):7741-5,1989
2. Sugimura K, et al. Biochem Biophys Res Commun. 247(2):466-72, 1998
3. Rieder MJ, et al. Nat Genet. 22(1):59-62, 1999
4. Natesh R, et al. Nature. 421(6922):551-4, 2003
5. Natesh R, et al. Biochemistry. 43(27):8718-24, 2004
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