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Actin AntibodyRabbit Monoclonal Antibody
| Country | United States
Ordering Information
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| Catalog # | Size | Availability | Price | |
| AJ1012a | 100ul 400 ul | 2-3 days | $ 315.00 | DISCONTINED INQUIRE CLICK INQUIRE Add to cart |
- Specification
- Citiations : 0
- Reviews
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Actin Antibody - Product info | |
| Application | WB, IHC, IF
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| Primary Accession | P68032 |
| Reactivity | Human, Mouse, Rat |
| Clone Names | EP184E |
| Calculated MW | 42019 Da |
| Gene ID 70 | |
| Other Names ACTC1, ACTC, Actin, alpha cardiac muscle 1, Alpha-cardiac actin | |
| Target/Specificity A synthetic peptide corresponding to residues near the N-terminus of human Actin was used as an immunogen. This Actin antibody recognizes all muscle actins. | |
| Dilution WB~~1:10000~20000 IHC~~1:100~200 | |
| Format 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. | |
| Storage Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. | |
| Precautions Actin Antibody is for research use only and not for use in diagnostic or therapeutic procedures. | |
Actin Antibody - Protein Information | |
| Name ACTC1 | |
| Synonyms ACTC | |
| Function Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells | |
| Cellular Location Cytoplasm, cytoskeleton. | |
Actin Antibody - Related products
AP1491a: Actin (ACTB/ACTC) Antibody (N-term)
AP1491c: Actin (ACTB/ACTC) Antibody (Center)
RI10087: ACTC1 predesign siRNA
LY10836a: ACTC1 Over-expression Lysate
BP1491a: ACTB/ACTC Antibody (N-term) Blocking Peptide
Actin Antibody - Application data
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A. Western blot analysis on A431 cell lysate using anti-Actin RabMAb (Cat. #AJ1012a), dilution 1:1,000
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B. Immunohistochemical staining of paraffin-embedded human cardiac muscle using anti-Actin RabMAb (Cat. #AJ1012a).
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C. Immunohistochemical analysis of paraffin-embedded human skeletal muscle using anti-Actin (Pan) RabMAb (Cat. #AJ1012a).
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D. Immunofluorescent staining of HeLa cells using anti-Actin RabMAb (Cat. #AJ1012a).
Actin Antibody - Research Areas
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BACKGROUND
Actins are highly conserved proteins ubiquitously expressed in all eukaryotic cells. They comprise, along with microtubules, a major component of the cytoskeleton. Actin has been found to be expressed in at least six isomeric forms. It is expressed in heart and skeletal striated muscle tissue, and in certain smooth muscle tissues, regulating contractile potentials for these cells. It is also expressed in the cytoplasm of non-muscle cells, functioning to control cell structure and motility (1). Polymerization of globular actin (G-actin) leads to a structural filament (F-actin), whose formation requires the Arp2/3 complex (2). It has been reported that actin is hyperphosphorylated in primary breast tumors (3). It has also been shown that actin dysfunction leads to certain types of heart failure (4).
REFERENCES
1. Herman, I.M. Curr. Opin. Cell Biol. 5: 48?55, 1993. 2. Bailly, M., et al. Curr Biol. 11: 620?5, 2001. 3. Lim, Y.P., et al. Clin. Cancer Res. 10: 3980?3987, 2004. 4. Olson, T.M., et al. Science 280: 750?2, 1998.
