|Application ||WB, IHC, IF|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||42019 Da|
|Other Names||Actin, alpha cardiac muscle 1, Alpha-cardiac actin, ACTC1, ACTC|
|Target/Specificity||A synthetic peptide corresponding to residues near the N-terminus of human Actin was used as an immunogen. This Actin antibody recognizes all muscle actins.|
|Format||50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||Actin Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.|
|Cellular Location||Cytoplasm, cytoskeleton.|
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Provided below are standard protocols that you may find useful for product applications.
Actins are highly conserved proteins ubiquitously expressed in all eukaryotic cells. They comprise, along with microtubules, a major component of the cytoskeleton. Actin has been found to be expressed in at least six isomeric forms. It is expressed in heart and skeletal striated muscle tissue, and in certain smooth muscle tissues, regulating contractile potentials for these cells. It is also expressed in the cytoplasm of non-muscle cells, functioning to control cell structure and motility (1). Polymerization of globular actin (G-actin) leads to a structural filament (F-actin), whose formation requires the Arp2/3 complex (2). It has been reported that actin is hyperphosphorylated in primary breast tumors (3). It has also been shown that actin dysfunction leads to certain types of heart failure (4).
1. Herman, I.M. Curr. Opin. Cell Biol. 5: 48?55, 1993. 2. Bailly, M., et al. Curr Biol. 11: 620?5, 2001. 3. Lim, Y.P., et al. Clin. Cancer Res. 10: 3980?3987, 2004. 4. Olson, T.M., et al. Science 280: 750?2, 1998.
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