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Actin Antibody

Rabbit Monoclonal Antibody

     
  • WB - Actin Antibody AJ1012a
    A. Western blot analysis on A431 cell lysate using anti-Actin RabMAb (Cat. #AJ1012a), dilution 1:1,000
  • IHC - Actin Antibody AJ1012a
    B. Immunohistochemical staining of paraffin-embedded human cardiac muscle using anti-Actin RabMAb (Cat. #AJ1012a).
  • IHC - Actin Antibody AJ1012a
    C. Immunohistochemical analysis of paraffin-embedded human skeletal muscle using anti-Actin (Pan) RabMAb (Cat. #AJ1012a).
  • IF - Actin Antibody AJ1012a
    D. Immunofluorescent staining of HeLa cells using anti-Actin RabMAb (Cat. #AJ1012a).
  • SPECIFICATION
  • CITATIONS
  • PROTOCOLS
  • BACKGROUND
Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC, IF
Primary Accession P68032
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Monoclonal
Clone Names EP184E
Calculated MW 42019 Da
Gene ID 70
Other Names Actin, alpha cardiac muscle 1, Alpha-cardiac actin, ACTC1, ACTC
Target/Specificity A synthetic peptide corresponding to residues near the N-terminus of human Actin was used as an immunogen. This Actin antibody recognizes all muscle actins.
Dilution WB~~1:10000~20000
IHC~~1:100~200
Format 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsActin Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name ACTC1
Synonyms ACTC
Function Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
Cellular Location Cytoplasm, cytoskeleton.
Research Areas
Citations (0)

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Background

Actins are highly conserved proteins ubiquitously expressed in all eukaryotic cells. They comprise, along with microtubules, a major component of the cytoskeleton. Actin has been found to be expressed in at least six isomeric forms. It is expressed in heart and skeletal striated muscle tissue, and in certain smooth muscle tissues, regulating contractile potentials for these cells. It is also expressed in the cytoplasm of non-muscle cells, functioning to control cell structure and motility (1). Polymerization of globular actin (G-actin) leads to a structural filament (F-actin), whose formation requires the Arp2/3 complex (2). It has been reported that actin is hyperphosphorylated in primary breast tumors (3). It has also been shown that actin dysfunction leads to certain types of heart failure (4).

References

1. Herman, I.M. Curr. Opin. Cell Biol. 5: 48?55, 1993. 2. Bailly, M., et al. Curr Biol. 11: 620?5, 2001. 3. Lim, Y.P., et al. Clin. Cancer Res. 10: 3980?3987, 2004. 4. Olson, T.M., et al. Science 280: 750?2, 1998.

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Discontinued
Cat# AJ1012a
Size:
(40 western blots)
Alternative Products:

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