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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   Adducin alpha Antibody (C-term)   

Adducin alpha Antibody (C-term)

Rabbit Monoclonal Antibody

     
  • WB - Adducin alpha Antibody (C-term) AJ1015a
    A. Western blot analysis on Hela cell lysate using anti-K562 RabMAb (Cat. #AJ1015a), dilution 1:50,000.
  • IHC - Adducin alpha Antibody (C-term) AJ1015a
    B. Immunohistochemical analysis of paraffin-embedded human lung using anti-Adducin (C-term) RabMAb (Cat. #AJ1015a).
  • IF - Adducin alpha Antibody (C-term) AJ1015a
    C. Immunofluorescent staining of HeLa using anti-Adducin RabMAb (C-term) (Cat. #AJ1015a).
  • SPECIFICATION
  • CITATIONS
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC, IF
Primary Accession P35611
Reactivity Human, Mouse
Host Rabbit
Clonality Monoclonal
Clone Names EP734Y
Calculated MW 80955 Da
Gene ID 118
Other Names Alpha-adducin, Erythrocyte adducin subunit alpha, ADD1, ADDA
Target/Specificity A synthetic peptide corresponding to residues in the C-term of human Adducin was used as immunogen.
Dilution WB~~1:500~1000
IHC~~1:250~500
Format 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsAdducin alpha Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name ADD1
Synonyms ADDA
Function Membrane-cytoskeleton-associated protein that promotes the assembly of the spectrin-actin network. Binds to calmodulin.
Cellular Location Cytoplasm, cytoskeleton. Cell membrane; Peripheral membrane protein; Cytoplasmic side
Tissue Location Expressed in all tissues. Found in much higher levels in reticulocytes than the beta subunit
Research Areas
Citations (0)

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Background

Adducin is a membrane skeletal protein that binds to actin filaments (F-actin) and promotes the association of spectrin with F-actin to form a spectrin-actin meshwork beneath plasma membranes (1-2). Adducin is a heterodimeric protein that consists of related subunits; α and β, or α and γ subunits (3). Adducin binds with high affinity to Ca(2+)/calmodulin and is a substrate for protein kinase C (PKC), and protein kinase A (PKA) (4). Phosphorylation of adducing alpha by Rho-kinase at Thr445 and Thr480 enhances the F-actin-binding activity of adducing alpha (5).

References

1. Gardner, K.; Bennett, V. : J. Biol. Chem. 261: 1339-1348, 1986.
2. Kuhlman, P.A., C.A. Hughes, V. Bennett, and V.M. Fowler. 1996. J. Biol. Chem. 271: 7986-7991
3. Dong, L., C. Chapline, B. Mousseau, L. Fowler, K. Ramsay, J.L. Stevens, and S. Jaken. 1995. J. Biol. Chem. 270: 25534-25540
4. Palfrey, H. C., Waseem, A. (1985) J. Biol. Chem. 260, 16021-16029
5. Fukata et al. J. Cell Biol., Volume 145, Number 2, April 19, 1999 347-361

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Discontinued
Cat# AJ1015a
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