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AE1 Antibody Phospho (pY359)

Rabbit Monoclonal Antibody

  • WB - AE1 Antibody Phospho (pY359) AJ1018a
    A. Western blot analysis on red blood cell lysate using anti-Phospho-AE1 (pY359) RabMAb (Cat. #AJ1018a), 1:50,000 dilution. Cells were either (A) untreated (B) treated with pervanadate.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P02730
Reactivity Human
Host Rabbit
Clonality Monoclonal
Clone Names EPR1427Y
Calculated MW 101792 Da
Gene ID 6521
Other Names Band 3 anion transport protein, Anion exchange protein 1, AE 1, Anion exchanger 1, Solute carrier family 4 member 1, CD233, SLC4A1, AE1, DI, EPB3
Target/Specificity A phospho specific peptide corresponding to residues surrounding tyrosine 359 of human AE1 was used as an immunogen.
Dilution WB~~1:50000
Format 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsAE1 Antibody Phospho (pY359) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name SLC4A1
Synonyms AE1, DI, EPB3
Function Functions both as a transporter that mediates electroneutral anion exchange across the cell membrane and as a structural protein. Major integral membrane glycoprotein of the erythrocyte membrane; required for normal flexibility and stability of the erythrocyte membrane and for normal erythrocyte shape via the interactions of its cytoplasmic domain with cytoskeletal proteins, glycolytic enzymes, and hemoglobin. Functions as a transporter that mediates the 1:1 exchange of inorganic anions across the erythrocyte membrane. Mediates chloride-bicarbonate exchange in the kidney, and is required for normal acidification of the urine.
Cellular Location Cell membrane; Multi-pass membrane protein. Basolateral cell membrane; Multi-pass membrane protein Note=Detected in the erythrocyte cell membrane and on the basolateral membrane of alpha-intercalated cells in the collecting duct in the kidney
Tissue Location Detected in erythrocytes (at protein level). Erythrocytes.
Research Areas
Citations (0)

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The AE1 (anion exchanger, band 3) protein is expressed in erythrocytes and in the A-type intercalated cells of the kidney distal collecting tubule. In both cell types it mediates the electroneutral transport of chloride and bicarbonate ions across the lipid bilayer, and, in erythrocytes (1). AE1 is phosphorylated on Tyr359 and Tyr404 by Lyn in erythrocytes. Phosphorylation of these residues is necessary for correct localization and recycling of kidney AE1 to the basolateral membrane (2).


1. Sahr KE, et al. Genomics 24(3):491-501, 1994
2. Toye AM et al. Biochem Soc Symp 72:47-63, 2005

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Cat# AJ1018a
(40 western blots)

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