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AFP Antibody (C-term)

Rabbit Monoclonal Antibody

     
  • WB - AFP Antibody (C-term) AJ1019a
    A. Western blot analysis on HepG2 membrane using anti-AFP RabMAb (Cat. #AJ1019a), dilution 1:50.000
  • IHC - AFP Antibody (C-term) AJ1019a
    B. Immunohistochemical staining of paraffin-embedded human fetal liver using anti-AFP RabMAb (Cat. #AJ1019a).
  • SPECIFICATION
  • CITATIONS: 1
  • PROTOCOLS
  • BACKGROUND
Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC
Primary Accession P02771
Reactivity Human
Host Rabbit
Clonality Monoclonal
Clone Names EP1017Y
Calculated MW 68678 Da
Gene ID 174
Other Names Alpha-fetoprotein, Alpha-1-fetoprotein, Alpha-fetoglobulin, AFP, HPAFP
Target/Specificity A synthetic peptide corresponding to residues near the C-term of human AFP was used as an immunogen.
Dilution WB~~1:1000~2000
IHC~~1:250~500
Format 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsAFP Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name AFP
Synonyms HPAFP
Function Binds copper, nickel, and fatty acids as well as, and bilirubin less well than, serum albumin. Only a small percentage (less than 2%) of the human AFP shows estrogen-binding properties.
Cellular Location Secreted.
Tissue Location Plasma. Synthesized by the fetal liver and yolk sac
Research Areas
Citations ( 0 )

Background

Alpha-fetoprotein (AFP) is a serum glycoprotein protein produced in the liver or yolk sac of fetal staged mammals. AFP synthesis is minimal after birth and trace amount is expressed in the adult liver (1). AFP gene expression is regulated by the interactions between steroid hormone receptors and transcriptional factors in separate signal transduction pathways (2). AFP functions as a binding and transporting ligand and cell growth regulator. Elevated expression level of AFP has been implicated in colorectal, ovarian, pancreatic, testicular, and certain liver cancers. High levels of AFP is also seen in some diseases such as hepatitis and colitis. AFP is used as a screening marker for fetal abnormalities in pregnant women such as Down syndrome. Recently, AFP has been introduced as an anti-cancer drugs-ligand carrier, transporting drugs to target tumor cells, increasing anti-tumor efficiency (3).

References

1. Wang XW, et al. World J Gastroenterol 7:345-351, 2001.
2. Chen H, et al Crit Rev Eukaryot Gene Expr, 7:11-41, 1997.
3. Zheng AI, et al. CJC 22:108-111, 2003.

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Discontinued
Cat# AJ1019a
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