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AIF AntibodyRabbit Monoclonal Antibody
| Country | United States
Ordering Information
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| Catalog # | Size | Availability | Price | |
| AJ1021a | 100ul 400 ul | 2-3 days | $ 315.00 | DISCONTINED INQUIRE CLICK INQUIRE Add to cart |
- Specification
- Citiations : 0
- Reviews
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- Backgrounds
AIF Antibody - Product info | |
| Application | WB, IHC, IF, FC
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| Primary Accession | O95831 |
| Reactivity | Human, Mouse, Rat |
| Clone Names | E20 |
| Calculated MW | 66901 Da |
| Gene ID 9131 | |
| Other Names AIFM1, AIF, PDCD8, Apoptosis-inducing factor 1, mitochondrial, Programmed cell death protein 8 | |
| Target/Specificity A synthetic peptide corresponding to residues near the C-terminus of Human AIF was used as immunogen. | |
| Dilution WB~~1:2500 IHC~~1:500 | |
| Format 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. | |
| Storage Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. | |
| Precautions AIF Antibody is for research use only and not for use in diagnostic or therapeutic procedures. | |
AIF Antibody - Protein Information | |
| Name AIFM1 | |
| Synonyms AIF, PDCD8 | |
| Function Probable oxidoreductase that has a dual role in controlling cellular life and death; during apoptosis, it is translocated from the mitochondria to the nucleus to function as a proapoptotic factor in a caspase-independent pathway, while in normal mitochondria, it functions as an antiapoptotic factor via its oxidoreductase activity. The soluble form (AIFsol) found in the nucleus induces 'parthanatos' i.e. caspase-independent fragmentation of chromosomal DNA. Interacts with EIF3G,and thereby inhibits the EIF3 machinery and protein synthesis, and activates casapse-7 to amplify apoptosis. Plays a critical role in caspase- independent, pyknotic cell death in hydrogen peroxide-exposed cells. Binds to DNA in a sequence-independent manner | |
| Cellular Location Mitochondrion intermembrane space. Mitochondrion inner membrane. Cytoplasm. Nucleus. Cytoplasm, perinuclear region. Note=Proteolytic cleavage during or just after translocation into the mitochondrial intermembrane space (IMS) results in the formation of an inner-membrane-anchored mature form (AIFmit). During apoptosis, further proteolytic processing leads to a mature form, which is confined to the mitochondrial IMS in a soluble form (AIFsol). AIFsol is released to the cytoplasm in response to specific death signals, and translocated to the nucleus, where it induces nuclear apoptosis. Colocalizes with EIF3G in the nucleus and perinuclear region | |
| Tissue Location Isoform 5 is frequently down-regulated in human cancers | |
AIF Antibody - Related products
AP4871b: TXNDC12 Antibody (C-term)
AP8910a: AIFM1 Antibody (N-term)
RI10187: AIFM1 predesign siRNA
RI15546: TXNDC12 predesign siRNA
LY12008a: AIFM1 Over-expression Lysate
BP4871b: TXNDC12 Antibody (C-term) Blocking Peptide
AIF Antibody - Application data
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A. Western blot analysis of K562 cell lysate using anti-AIF RabMAb (Cat. #AJ1021a), 1:1,000 dilution.
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B. Immunohistochemical analysis of paraffin-embedded human cervical carcinoma using anti-AIF RabMAb (Cat. #AJ1021a).
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C. Immunofluorescent staining of HeLa cells, using AIF RabMAb. (Green fluorescence- AIF localized in mitochondria, Red - PI nuclear staining). (Cat. #AJ1021a).
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D. Flow cytometric analysis of permeabilized K562 cells using anti-AIF RabMAb (red) (Cat. #AJ1021a) or a rabbit IgG (negative)(green).
AIF Antibody - Research Areas
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BACKGROUND
AIF, or apoptosis-inducing factor, is a flavoprotein localized to the mitochondrial membrane that plays a primary role in caspase-independent cell death, or apoptosis (1,2). AIF translocates to the nucleus when apoptosis is induced and causes mitochondria to release the apoptogenic proteins, cytochrome c and caspase-9. Apoptotic activity of AIF leads to chromatin condensation and DNA fragmentation (1). Similar to cytochrome c, AIF is a bifunctional protein with an oxidoreductase function and a second apoptogenic function. In contrast to cytochrome c, however, AIF acts in a caspase-independent fashion (2,3,4).
REFERENCES
1. Susin, S.A. et al. Molecular characterization of mitochondrial apoptosis-inducing factor. Nature 397(6718): 441
2. Daugas, E. et al. Apoptosis-inducing factor (AIF): a ubiquitous mitochondrial oxidoreductase involved in apoptosis. FEBS Lett. 476(3): 118
3. Daugas, E. et al. Mitochondrio-nuclear translocation of AIF in apoptosis and necrosis. FASEB J. 14(5):729
4. Susin, S.A. et al. Two distinct pathways leading to nuclear apoptosis. J. Exp. Med. 192(4): 571
