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AIF Antibody

Rabbit Monoclonal Antibody

  • WB - AIF Antibody AJ1021a
    A. Western blot analysis of K562 cell lysate using anti-AIF RabMAb (Cat. #AJ1021a), 1:1,000 dilution.
  • IHC - AIF Antibody AJ1021a
    B. Immunohistochemical analysis of paraffin-embedded human cervical carcinoma using anti-AIF RabMAb (Cat. #AJ1021a).
  • IF - AIF Antibody AJ1021a
    C. Immunofluorescent staining of HeLa cells, using AIF RabMAb. (Green fluorescence- AIF localized in mitochondria, Red - PI nuclear staining). (Cat. #AJ1021a).
  • FC - AIF Antibody AJ1021a
    D. Flow cytometric analysis of permeabilized K562 cells using anti-AIF RabMAb (red) (Cat. #AJ1021a) or a rabbit IgG (negative)(green).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession O95831
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Monoclonal
Clone Names E20
Calculated MW 66901 Da
Gene ID 9131
Other Names Apoptosis-inducing factor 1, mitochondrial, 111-, Programmed cell death protein 8, AIFM1, AIF, PDCD8
Target/Specificity A synthetic peptide corresponding to residues near the C-terminus of Human AIF was used as immunogen.
Dilution WB~~1:2500
Format 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsAIF Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name AIFM1
Synonyms AIF, PDCD8
Function Functions both as NADH oxidoreductase and as regulator of apoptosis. In response to apoptotic stimuli, it is released from the mitochondrion intermembrane space into the cytosol and to the nucleus, where it functions as a proapoptotic factor in a caspase-independent pathway. In contrast, functions as an antiapoptotic factor in normal mitochondria via its NADH oxidoreductase activity. The soluble form (AIFsol) found in the nucleus induces 'parthanatos' i.e. caspase-independent fragmentation of chromosomal DNA. Interacts with EIF3G,and thereby inhibits the EIF3 machinery and protein synthesis, and activates casapse-7 to amplify apoptosis. Plays a critical role in caspase- independent, pyknotic cell death in hydrogen peroxide-exposed cells. Binds to DNA in a sequence-independent manner.
Cellular Location Mitochondrion intermembrane space. Mitochondrion inner membrane. Cytoplasm. Nucleus. Cytoplasm, perinuclear region. Note=Proteolytic cleavage during or just after translocation into the mitochondrial intermembrane space (IMS) results in the formation of an inner-membrane-anchored mature form (AIFmit). During apoptosis, further proteolytic processing leads to a mature form, which is confined to the mitochondrial IMS in a soluble form (AIFsol). AIFsol is released to the cytoplasm in response to specific death signals, and translocated to the nucleus, where it induces nuclear apoptosis. Colocalizes with EIF3G in the nucleus and perinuclear region Isoform 5: Cytoplasm
Tissue Location Detected in muscle and skin fibroblasts (at protein level). Isoform 5 is frequently down-regulated in human cancers.
Research Areas
Citations (0)

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AIF, or apoptosis-inducing factor, is a flavoprotein localized to the mitochondrial membrane that plays a primary role in caspase-independent cell death, or apoptosis (1,2). AIF translocates to the nucleus when apoptosis is induced and causes mitochondria to release the apoptogenic proteins, cytochrome c and caspase-9. Apoptotic activity of AIF leads to chromatin condensation and DNA fragmentation (1). Similar to cytochrome c, AIF is a bifunctional protein with an oxidoreductase function and a second apoptogenic function. In contrast to cytochrome c, however, AIF acts in a caspase-independent fashion (2,3,4).


1. Susin, S.A. et al. Molecular characterization of mitochondrial apoptosis-inducing factor. Nature 397(6718): 441
2. Daugas, E. et al. Apoptosis-inducing factor (AIF): a ubiquitous mitochondrial oxidoreductase involved in apoptosis. FEBS Lett. 476(3): 118
3. Daugas, E. et al. Mitochondrio-nuclear translocation of AIF in apoptosis and necrosis. FASEB J. 14(5):729
4. Susin, S.A. et al. Two distinct pathways leading to nuclear apoptosis. J. Exp. Med. 192(4): 571

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Cat# AJ1021a
(40 western blots)
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