|Reactivity||Human, Mouse, Rat|
|Calculated MW||284539 Da|
|Other Names||Spectrin alpha chain, non-erythrocytic 1, Alpha-II spectrin, Fodrin alpha chain, Spectrin, non-erythroid alpha subunit, SPTAN1, NEAS, SPTA2|
|Target/Specificity||A synthetic peptide corresponding to residues in human Alpha Fodrin was used as an immunogen.|
|Format||50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||Alpha Fodrin Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Fodrin, which seems to be involved in secretion, interacts with calmodulin in a calcium-dependent manner and is thus candidate for the calcium-dependent movement of the cytoskeleton at the membrane.|
|Cellular Location||Cytoplasm, cytoskeleton. Cytoplasm, cell cortex. Note=Expressed along the cell membrane in podocytes and presumptive tubule cells during glomerulogenesis and is expressed along lateral cell margins in tubule cells.|
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Provided below are standard protocols that you may find useful for product applications.
Alpha Fodrin is a member of the spectrin family of widely distributed filamentous proteins that form a supporting and organizing scaffold for cell membranes (1). Non-erythroid alpha spectrin, also called Alpha Fodrin, is a major cortical cytoskeletal protein that has been implicated in the establishment of specialized membrane- cytoskeletal domains in differentiating cells (2-3). Cleavage of Alpha Fodrin accompanies apoptosis, induced by activation via the CD3/T cell receptor complex, ligation of the Fas (CD95) molecule and other Fas-expressing cells, or treatment of cells with staurosporine, dexamethasone, or synthetic ceramide (4). The major products of the cleavage are amino-terminal (135 kDa) and carboxy-terminal (150 kDa and 120 kDa) fragments. Antibodies against Alpha Fodrin are observed in diseases characterized by chronic apoptosis, and are valuable in the evaluation fo Sicca- and Sj鰃ren's syndrome (5-6).
1. Leto TL, et al. Mol Cell Biol. 8(1):1-9, 1988
2. Simonovic M, et al. J Biol Chem. 281(45):34333-40, 2006
3. DH Giebelhaus, et al. The Journal of Cell Biology 105:843-853,1987
4. Martin SJ, et al. J Biol Chem. 270(12):6425-8, 1995
5. Ulbricht K U, et al. Autoimmunity Reviews 2(2):109-113,2003
6. Kahaly, G. J., et al. Clinical & Experimental Immunology 140:166-172(7), 2005
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