- CITATIONS: 1
|Reactivity||Human, Mouse, Rat|
|Calculated MW||103058 Da|
|Other Names||Alpha-actinin-1, Alpha-actinin cytoskeletal isoform, F-actin cross-linking protein, Non-muscle alpha-actinin-1, ACTN1|
|Target/Specificity||A synthetic peptide corresponding to residues on the N-terminus of human Alpha-Actinin 1 was used as an immunogen.|
|Format||50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||Alpha-Actinin-1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||F-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. This is a bundling protein.|
|Cellular Location||Cytoplasm, cytoskeleton Cytoplasm, myofibril, sarcomere, Z line. Cell membrane. Cell junction. Cell projection, ruffle Note=Colocalizes with MYOZ2 and PPP3CA at the Z-line of heart and skeletal muscle. Colocalizes with PSD in membrane ruffles and central reticular structures (By similarity).|
Provided below are standard protocols that you may find useful for product applications.
Alpha-actinin is a ubiquitously expressed protein found in numerous actin structures. It consists of an N-terminal actin binding domain, a central rod domain, and a C-terminal domain and functions as a homodimer to cross-link actin filaments. The rod domain determines the distance between cross-linked actin filaments and also serves as an interaction site for several cytoskeletal and signaling proteins (1). Alpha-actinins are a multigene family of four actin-binding proteins related to dystrophin. Alpha-actinin-1 and -4 (ACTN1, ACTN4) are non-muscle isoforms version of ACTN. These isoform are commonly expressed in filopodia, lamellipodia, stress fibers, sites of adhesion and intercellular contacts (2).
1. Ylanne J, et al. Structure 9(7):597-604, 2001
2. Otey, CA and Carpen O. Cell Motil. Cytoskeleton 58:104-111, (2004).
If you have used an Abgent product and would like to share how it has performed, please click on the "Submit Review" button and provide the requested information. Our staff will examine and post your review and contact you if needed.
If you have any additional inquiries please email technical services at email@example.com.