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Alpha-Actinin-2 AntibodyRabbit Monoclonal Antibody
| Country | United States
Ordering Information
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| Catalog # | Size | Availability | Price | |
| AJ1030a | 100ul 400 ul | 2-3 days | $ 315.00 | DISCONTINED INQUIRE CLICK INQUIRE Add to cart |
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- Citiations : 0
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- Backgrounds
Alpha-Actinin-2 Antibody - Product info | |
| Application | WB, IHC
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| Primary Accession | P35609 |
| Reactivity | Human, Mouse, Rat |
| Clone Names | EP2529Y |
| Calculated MW | 103854 Da |
| Gene ID 88 | |
| Other Names ACTN2, Alpha-actinin-2, Alpha-actinin skeletal muscle isoform 2;F-actin cross-linking protein | |
| Target/Specificity A synthetic peptide corresponding to residues on the N-terminus of human Alpha-Actinin 2 was used as an immunogen. | |
| Dilution WB~~1:200000~1000000 IHC~~1:100~250 | |
| Format 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. | |
| Storage Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. | |
| Precautions Alpha-Actinin-2 Antibody is for research use only and not for use in diagnostic or therapeutic procedures. | |
Alpha-Actinin-2 Antibody - Protein Information | |
| Name ACTN2 | |
| Function F-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. This is a bundling protein | |
| Cellular Location Cytoplasm, myofibril, sarcomere, Z line. Note=Colocalizes with MYOZ1 and FLNC at the Z-lines of skeletal muscle | |
| Tissue Location Expressed in both skeletal and cardiac muscle. | |
Alpha-Actinin-2 Antibody - Related products
Alpha-Actinin-2 Antibody - Application data
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A. Western blot analysis on (A) Human skeletal muscle (B) rat heart (C) human heart lysate using anti-alpha-actinin 2 RabMAb (Cat. #AJ1030a).
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B. Immunohistochemical analysis of paraffin-embedded human muscles using anti-Alpha-Actinin 2 RabMAb (Cat. #AJ1030a).
Alpha-Actinin-2 Antibody - Research Areas
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BACKGROUND
Alpha-actinin is a ubiquitously expressed protein found in numerous actin structures. It consists of an N-terminal actin binding domain, a central rod domain, and a C-terminal domain and functions as a homodimer to cross-link actin filaments. The rod domain determines the distance between cross-linked actin filaments and also serves as an interaction site for several cytoskeletal and signaling proteins (1). Alpha-actinins are a multigene family of four actin-binding proteins related to dystrophin. The two skeletal muscle isoforms of alpha-actinin (ACTN2 and ACTN3) are major structural components of the Z-line involved in anchoring the actin-containing thin filaments. In humans, ACTN2 is expressed in all muscle fibers, while ACTN3 expression is restricted to a subset of type 2 fibers (2).
REFERENCES
1. Ylanne J, et al. Structure 9(7):597-604, 2001
2. Mills M et al. Hum Mol Genet 10(13): 1335-46, 2001
