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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   Alpha-Actinin-2 Antibody   

Alpha-Actinin-2 Antibody

Rabbit Monoclonal Antibody

  • WB - Alpha-Actinin-2 Antibody AJ1030b
    A. Western blot analysis on human skeletal muscle lysate using anti-Alpha-Actinin 2 RabMAb (Cat. #AJ1030b), dilution 1:50,000
  • IHC - Alpha-Actinin-2 Antibody AJ1030b
    B. Immunohistochemical analysis of paraffin-embedded human muscles using anti-Actinin 2 RabMAb (Cat. #AJ1030b).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P35609
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Monoclonal
Clone Names EP2530Y
Calculated MW 103854 Da
Gene ID 88
Other Names Alpha-actinin-2, Alpha-actinin skeletal muscle isoform 2, F-actin cross-linking protein, ACTN2
Target/Specificity A synthetic peptide corresponding to residues near the C-terminus of human Alpha-Actinin 2 was used as an immunogen.
Dilution WB~~1:1000~10000
Format 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsAlpha-Actinin-2 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name ACTN2
Function F-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. This is a bundling protein.
Cellular Location Cytoplasm, myofibril, sarcomere, Z line Note=Colocalizes with MYOZ1 and FLNC at the Z-lines of skeletal muscle
Tissue Location Expressed in both skeletal and cardiac muscle.
Research Areas
Citations (0)

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Alpha-actinin is a ubiquitously expressed protein found in numerous actin structures. It consists of an N-terminal actin binding domain, a central rod domain, and a C-terminal domain and functions as a homodimer to cross-link actin filaments. The rod domain determines the distance between cross-linked actin filaments and also serves as an interaction site for several cytoskeletal and signaling proteins (1). Alpha-actinins are a multigene family of four actin-binding proteins related to dystrophin. The two skeletal muscle isoforms of alpha-actinin (ACTN2 and ACTN3) are major structural components of the Z-line involved in anchoring the actin-containing thin filaments. In humans, ACTN2 is expressed in all muscle fibers, while ACTN3 expression is restricted to a subset of type 2 fibers (2).


1. Tsukada, S., et al (1993) Cell 72, 279-290
2. Rawlings, DJ et al. Immunol. 7, 237-246
3. Vassilev, AZ et al.. 1999. J. Biol. Chem. 274:1646-1656
4. Mahajan S, et al. Mol Cell Biol. 1995;15:5304

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Cat# AJ1030b
(40 western blots)
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