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Alpha-Tubulin Antibody

Rabbit Monoclonal Antibody

  • IF - Alpha-Tubulin Antibody AJ1034a
    C. Immunofluorescent staining of HeLa cells using anti- alpha-Tubulin RabMAb (Cat. #AJ1034a)
  • IHC - Alpha-Tubulin Antibody AJ1034a
    B. Immunohistochemical analysis of paraffin-embedded human gastric carcinoma using anti- alpha-Tubulin RabMAb (Cat. #AJ1034a).
  • WB - Alpha-Tubulin Antibody AJ1034a
    A. Western blot analysis on HeLa cell lysate using anti- alpha-Tubulin RabMAb (Cat. #AJ1034a), dilution 1:50,000.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P68363
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Monoclonal
Clone Names EP1332Y
Calculated MW 50152 Da
Gene ID 10376
Other Names Tubulin alpha-1B chain, Alpha-tubulin ubiquitous, Tubulin K-alpha-1, Tubulin alpha-ubiquitous chain, TUBA1B
Target/Specificity A synthetic peptide corresponding to residues near the N-term of human ?-Tubulin was used as immunogen.
Dilution IHC~~1:250~500
Format 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsAlpha-Tubulin Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Function Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.
Cellular Location Cytoplasm, cytoskeleton.
Research Areas
Citations ( 0 )


Microtubules are essential parts in eukaryotic cell structures, transportation, and mitosis. It consists mainly of 2 soluble protein subunits, alpha and beta tubulin. Beta tubulin binds to alpha tubulin to form tubulin heterodimer which is post-translationally modified (1). The tubulin dimer complex binds to GTP and assembles onto the positive ends of microtubules. After incorporation into the microtubules, bound GTP is hydrolyzed by beta tubulin. The stability of the dimer in the microtubules is depended on presence of beta tubulin, where dimer with GTP bound beta-tubulin is stable to microtubule incorporation (2). For both type of tubulin, the carboxy-terminal tail is post-translationally modified to regulate associated protein binding at microtubule surface (3). A mutation on alpha tubulin has been linked to abnormal neuronal migration and neurodevelopmental disorders in humans (4).


1. Wang HW et al. Nature 435: 911-915, 2005.
2. Cleveland DW et al. Cell 20: 95-105, 1980.
3. Westermann S, Weber K, Nat. Rev. Mol. Cell Biol. 4:938 -947, 2003.
4. Keays D, et al. Cell 128:45-47, 2007.

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Cat# AJ1034a
(40 western blots)
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