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Alpha-Tubulin AntibodyRabbit Monoclonal Antibody
| Country | United States
Ordering Information
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| Catalog # | Size | Availability | Price | |
| AJ1034a | 100ul 400 ul | 2-3 days | $ 315.00 | DISCONTINED INQUIRE CLICK INQUIRE Add to cart |
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- Citiations : 0
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Alpha-Tubulin Antibody - Product info | |
| Application | IF, IHC, WB
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| Primary Accession | P68363 |
| Reactivity | Human, Mouse, Rat |
| Clone Names | EP1332Y |
| Calculated MW | 50152 Da |
| Gene ID 10376 | |
| Other Names TUBA1B, Tubulin alpha-1B chain, Tubulin alpha-ubiquitous chain;Alpha-tubulin ubiquitous;Tubulin K-alpha-1 | |
| Target/Specificity A synthetic peptide corresponding to residues near the N-term of human ?-Tubulin was used as immunogen. | |
| Dilution IHC~~1:250~500 WB~~1:50000~200000 | |
| Format 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. | |
| Storage Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. | |
| Precautions Alpha-Tubulin Antibody is for research use only and not for use in diagnostic or therapeutic procedures. | |
Alpha-Tubulin Antibody - Protein Information | |
| Name TUBA1B | |
| Function Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain | |
| Cellular Location Cytoplasm, cytoskeleton. | |
Alpha-Tubulin Antibody - Related products
AP8729c: TUBA1B Antibody (Center)
RI15525: TUBA1B predesign siRNA
LY10933a: TUBA1B Over-expression Lysate
BP8729c: TUBA1B Antibody (Center) Blocking Peptide
AT2589a: K-ALPHA-1 Antibody (monoclonal) (M01)
Alpha-Tubulin Antibody - Application data
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C. Immunofluorescent staining of HeLa cells using anti- alpha-Tubulin RabMAb (Cat. #AJ1034a)
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B. Immunohistochemical analysis of paraffin-embedded human gastric carcinoma using anti- alpha-Tubulin RabMAb (Cat. #AJ1034a).
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A. Western blot analysis on HeLa cell lysate using anti- alpha-Tubulin RabMAb (Cat. #AJ1034a), dilution 1:50,000.
Alpha-Tubulin Antibody - Research Areas
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BACKGROUND
Microtubules are essential parts in eukaryotic cell structures, transportation, and mitosis. It consists mainly of 2 soluble protein subunits, alpha and beta tubulin. Beta tubulin binds to alpha tubulin to form tubulin heterodimer which is post-translationally modified (1). The tubulin dimer complex binds to GTP and assembles onto the positive ends of microtubules. After incorporation into the microtubules, bound GTP is hydrolyzed by beta tubulin. The stability of the dimer in the microtubules is depended on presence of beta tubulin, where dimer with GTP bound beta-tubulin is stable to microtubule incorporation (2). For both type of tubulin, the carboxy-terminal tail is post-translationally modified to regulate associated protein binding at microtubule surface (3). A mutation on alpha tubulin has been linked to abnormal neuronal migration and neurodevelopmental disorders in humans (4).
REFERENCES
1. Wang HW et al. Nature 435: 911-915, 2005.
2. Cleveland DW et al. Cell 20: 95-105, 1980.
3. Westermann S, Weber K, Nat. Rev. Mol. Cell Biol. 4:938 -947, 2003.
4. Keays D, et al. Cell 128:45-47, 2007.
